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LumiSpin BB, DNA Isolation Spin Kit for Blood and Buccal Swab
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 11553 |
10 minipreps
|
– | 21 天 | |
| 21553 |
50 minipreps
|
$185 | 21 天 |
The kit is designed to isolate total (genomic, mitochondrial, and viral) DNA from biological samples using spin columns. The kit includes all the necessary components. First, cell lysis is performed using Lysis Solution BB, and Proteinase K. Lysis Solution BB allows selective DNA binding to the membrane in the column. In the following step, a series of washings of bound DNA is performed. Special formulations of Washing Solutions allow impurities to be completely removed from the column membrane. Finally, the genomic DNA is eluted with Elution Buffer or, if necessary, with deionized water.
Kit Specifications:
- Samples: whole blood, buccal swabs, leukocytes, cultured mammalian cells
- Operation time: 30 minutes
- Column binding capacity: 20 µg DNA
- DNA typical yield: whole blood (100 µL) – 1–3 µg, buccal swab – 0.5–2 µg, cultured cells (1 × 106) – 3–6 µg
- DNA quality: 30–50 kb in size, OD 260/280 1.7–1.8
The isolated DNA is suitable for PCR, restriction enzyme digestion, Southern blotting, preparing samples for Sanger sequencing and NGS, etc.
| 储存条件: | |
| 安全数据表（MSDS）: | 下载 |
| 储存时间（月: | 12 |
参考文献
- Ikonnikova, A.; Morozova, A.; Antonova, O.; Ochneva, A.; Fedoseeva, E.; Abramova, O.; Emelyanova, M.; Filippova, M.; Morozova, I.; Zorkina, Y.; Syunyakov, T.; Andryushchenko, A.; Andreuyk, D.; Kostyuk, G.; Gryadunov, D. Evaluation of the Polygenic Risk Score for Alzheimer’s Disease in Russian Patients with Dementia Using a Low-Density Hydrogel Oligonucleotide Microarray. International Journal of Molecular Sciences, 2023, 24(19), 14765. doi: 10.3390/ijms241914765
- Ikonnikova, A.; Rodina, T.; Dmitriev, A.; Melnikov, E.; Kazakov, R.; Nasedkina, T. The Influence of the CES1 Genotype on the Pharmacokinetics of Enalapril in Patients with Arterial Hypertension. Journal of Personalized Medicine, 2022, 12(4), 580. doi: 10.3390/jpm12040580
- Ikonnikova, A.; Kazakov, R.; Rodina, T.; Dmitriev, A.; Melnikov, E.; Zasedatelev, A.; Nasedkina, T. The Influence of Structural Variants of the CES1 Gene on the Pharmacokinetics of Enalapril, Presumably Due to Linkage Disequilibrium with the Intronic Rs2244613. Genes, 2022, 13(12), 2225. doi: 10.3390/genes13122225
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