活化酯
Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.
Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.
Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.
3-马来酰亚胺丙酸NHS-酯 (3-Maleimidopropionic acid NHS-ester)
具有胺反应性琥珀酰亚胺酯和巯基反应性的马来酰亚胺基团的双功能交联剂。| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 3419-100mg | 100 mg | – | 现货 | |
| 3419-500mg | 500 mg | $110 | 现货 |
Alkyne-PEG2-NHS ester
Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on diethyleneglycol (PEG2).| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2219-1g | 1 g |
$450
|
现货 | |
| 2219-5g | 5 g |
$980
|
现货 |
Alkyne-PEG3-NHS ester
Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2298-1g | 1 g |
$490
|
现货 | |
| 2298-5g | 5 g |
$980
|
现货 |
Alkyne-PEG4-NHS ester
Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on tetraethyleneglycol (PEG4).| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2409-100mg | 100 mg | $250 | 现货 | |
| 2409-1g | 1 g |
$790
|
现货 |
ATFB SE（N-琥珀酰亚胺基 4-叠氮四氟苯甲酸酯） [ATFB SE (N-Succinimidyl 4-Azidotetrafluorobenzoate)]
N-Succinimidyl 4-AzidotetrafluBenzate (ATFB SE, ATFB-OSu) 是一种光激活连接剂，是用于研究生物受体的通用标记试剂。| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2574-250mg | 250 mg | $134 | 现货 | |
| 2574-1g | 1 g |
$419
|
现货 |
Azide-PEG6-NHS ester
A hydrophilic bifunctional linker with azide and NHS ester function groups flanking PEG6 (hexaethylene glycol).| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 3692-250mg | 250 mg | $325 | 5 天 | |
| 3692-1g | 1 g |
$539
|
现货 |
叠氮基丁酸 NHS 酯 (Azidobutyric acid NHS ester)
活化的NHS 酯用于多肽和蛋白质的叠氮基标记。| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 53720 | 50 mg | $110 | 现货 | |
| 63720 | 100 mg | $210 | 现货 | |
| 73720 | 1 g |
$950
|
现货 | |
| 83720 | 5 g | 请查询 | 现货 |
Biotin-PEG4-NHS ester
PEG4-containing activated ester for simple and efficient biotin labeling of antibodies, proteins, and other primary amine-containing biomolecules.| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2893-100mg | 100 mg | $145 | 现货 | |
| 2893-1g | 1 g |
$590
|
现货 |
生物素-XX-NHS酯 (Biotin-XX-NHS ester)
生物素衍生物，长的间隔基可减少空间效应，可通过与伯胺反应与氨基酸、肽或蛋白质结合。| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2897-100mg | 100 mg | $225 | 现货 | |
| 2897-1g | 1 g |
$670
|
现货 |
DBCO-NHS 酯
偶氮二苯并环辛炔 (DBCO、ADIBO) 试剂用于应变促进的无铜点击化学 (SPAAC)。该试剂含有 NHS 酯官能团，用于将环辛炔连接到各种分子上。| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 34720 | 10 mg | $80 | 现货 | |
| 44720 | 25 mg | $110 | 现货 | |
| 54720 | 50 mg | $135 | 现货 | |
| 64720 | 100 mg |
$210
$178
|
现货 | |
| 94720 | 1 g |
$1000
$970
|
现货 | |
| K4720 | 5 g |
$3570
$2990
|
现货 |