活化酯

Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.

Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.

NHS ester coupling chemistry

Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.

3-马来酰亚胺丙酸NHS-酯 (3-Maleimidopropionic acid NHS-ester)

具有胺反应性琥珀酰亚胺酯和巯基反应性的马来酰亚胺基团的双功能交联剂。
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目录号 数量 价格 提前通知时间
3419-100mg 100 mg –   现货
3419-500mg 500 mg $110 现货
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Alkyne-PEG2-NHS ester

Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on diethyleneglycol (PEG2).
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目录号 数量 价格 提前通知时间
2219-1g 1 g $450 现货
2219-5g 5 g $980 现货
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Alkyne-PEG3-NHS ester

Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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目录号 数量 价格 提前通知时间
2298-1g 1 g $490 现货
2298-5g 5 g $980 现货
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Alkyne-PEG4-NHS ester

Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on tetraethyleneglycol (PEG4).
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目录号 数量 价格 提前通知时间
2409-100mg 100 mg $250 现货
2409-1g 1 g $790 现货
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ATFB SE(N-琥珀酰亚胺基 4-叠氮四氟苯甲酸酯) [ATFB SE (N-Succinimidyl 4-Azidotetrafluorobenzoate)]

N-Succinimidyl 4-AzidotetrafluBenzate (ATFB SE, ATFB-OSu) 是一种光激活连接剂,是用于研究生物受体的通用标记试剂。
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目录号 数量 价格 提前通知时间
2574-250mg 250 mg $134 现货
2574-1g 1 g $419 现货
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Azide-PEG6-NHS ester

A hydrophilic bifunctional linker with azide and NHS ester function groups flanking PEG6 (hexaethylene glycol).
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目录号 数量 价格 提前通知时间
3692-250mg 250 mg $325 5 天
3692-1g 1 g $539 现货
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叠氮基丁酸 NHS 酯 (Azidobutyric acid NHS ester)

活化的NHS 酯用于多肽和蛋白质的叠氮基标记。
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目录号 数量 价格 提前通知时间
53720 50 mg $110 现货
63720 100 mg $210 现货
73720 1 g $950 现货
83720 5 g 请查询 现货
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Biotin-PEG4-NHS ester

PEG4-containing activated ester for simple and efficient biotin labeling of antibodies, proteins, and other primary amine-containing biomolecules.
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目录号 数量 价格 提前通知时间
2893-100mg 100 mg $145 现货
2893-1g 1 g $590 现货
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生物素-XX-NHS酯 (Biotin-XX-NHS ester)

生物素衍生物,长的间隔基可减少空间效应,可通过与伯胺反应与氨基酸、肽或蛋白质结合。
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目录号 数量 价格 提前通知时间
2897-100mg 100 mg $225 现货
2897-1g 1 g $670 现货
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DBCO-NHS 酯

偶氮二苯并环辛炔 (DBCO、ADIBO) 试剂用于应变促进的无铜点击化学 (SPAAC)。该试剂含有 NHS 酯官能团,用于将环辛炔连接到各种分子上。
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目录号 数量 价格 提前通知时间
34720 10 mg $80 现货
44720 25 mg $110 现货
54720 50 mg $135 现货
64720 100 mg $210 $178 现货
94720 1 g $1000 $970 现货
K4720 5 g $3570 $2990 现货