活化酯

Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.

Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.

NHS ester coupling chemistry

Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.

(5,6)-FAM diacetate NHS ester

Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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目录号 数量 价格 提前通知时间
2934-1mg 1 mg –   现货
2934-25mg 25 mg $160 现货
2934-50mg 50 mg $270 现货
2934-100mg 100 mg $520 现货
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AF 343(香豆素)-X-NHS 酯 (AF 343 (Coumarin)-X-NHS ester)

AF 343(香豆素)是一种蓝光发射染料,可用作荧光素 (FAM) 的 FRET 供体。该结构的特点是香豆素 343 荧光团和官能团之间有一个连接体。它提供了更好的溶解性以及荧光团和官能团之间的空间分离。
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目录号 数量 价格 提前通知时间
12620 1 mg $110 现货
22620 5 mg $210 现货
42620 25 mg $410 现货
52620 50 mg $695 现货
62620 100 mg $1190 现货
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AF 430 NHS ester

AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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目录号 数量 价格 提前通知时间
10820 1 mg $110 现货
20820 5 mg $165 现货
40820 25 mg $380 现货
50820 50 mg $695 现货
60820 100 mg $1190 现货
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AF 488 NHS 酯 (AF 488 NHS ester)

AF 488 NHS 酯,一种明亮、光稳定、亲水性的绿色通道荧光染料。
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目录号 数量 价格 提前通知时间
11820 1 mg $125 现货
21820 5 mg $325 现货
31820 10 mg $480 现货
41820 25 mg $950 现货
51820 50 mg $1625 现货
61820 100 mg $2090 现货
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AF 488 TFP 酯 (AF 488 TFP ester)

绿色荧光染料 AF 488 的四氟苯酯,用于标记蛋白质、多肽和修饰寡核苷酸中的氨基。
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目录号 数量 价格 提前通知时间
118T0 1 mg $165 现货
218T0 5 mg $510 现货
418T0 25 mg $1650 现货
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AF 532 NHS 酯 (AF 532 NHS ester)

AF 532 染料的 NHS 酯,通过与氨基反应标记蛋白质、多肽和修饰寡核苷酸。
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目录号 数量 价格 提前通知时间
1C820 1 mg $140 现货
2C820 5 mg $450 现货
4C820 25 mg $1460 现货
5C820 50 mg $1890 现货
6C820 100 mg $2640 现货
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AF 555 NHS ester

AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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目录号 数量 价格 提前通知时间
1K820 1 mg $140 现货
2K820 5 mg $450 现货
4K820 25 mg $1460 现货
5K820 50 mg $1890 现货
6K820 100 mg $2640 现货
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AF 568 NHS ester

AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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目录号 数量 价格 提前通知时间
14820 1 mg $125 现货
24820 5 mg $325 现货
44820 25 mg $720 现货
54820 50 mg $1325 现货
64820 100 mg $2090 现货
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AF 594 NHS酯 (AF 594 NHS ester)

AF 594 染料的红色荧光 NHS-酯,用于标记蛋白质、肽、抗体和任何含有 -NH2 基团的分子。纯5-异构体
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目录号 数量 价格 提前通知时间
2825-1mg 1 mg $125 现货
2825-5mg 5 mg $425 现货
2825-25mg 25 mg $1240 现货
2825-50mg 50 mg $1890 现货
2825-100mg 100 mg $2640 现货