Activated esters

Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.

Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.

NHS ester coupling chemistry

Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.

(5,6)-FAM diacetate NHS ester

Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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目录号 数量 价格 提前通知时间
2934-1mg 1 mg –   现货
2934-25mg 25 mg $160 5 天
2934-50mg 50 mg $270 现货
2934-100mg 100 mg $520 现货
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AF 343 (Coumarin)-X-NHS ester

AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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目录号 数量 价格 提前通知时间
12620 1 mg $110 现货
22620 5 mg $210 现货
42620 25 mg $410 现货
52620 50 mg $695 现货
62620 100 mg $1190 现货
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AF 430 NHS ester

AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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目录号 数量 价格 提前通知时间
10820 1 mg $110 现货
20820 5 mg $165 现货
40820 25 mg $380 现货
50820 50 mg $695 现货
60820 100 mg $1190 现货
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AF 488 NHS 酯 (AF 488 NHS ester)

AF 488 NHS 酯,一种明亮、光稳定、亲水性的绿色通道荧光染料。
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目录号 数量 价格 提前通知时间
11820 1 mg $125 现货
21820 5 mg $325 现货
31820 10 mg $480 现货
41820 25 mg $950 现货
51820 50 mg $1625 现货
61820 100 mg $2090 现货
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AF 488 TFP ester

Tetrafluorophenyl ester of green fluorescent dye AF 488 for labeling amino groups in proteins, peptides, and modified oligonucleotides.
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目录号 数量 价格 提前通知时间
118T0 1 mg $165 现货
218T0 5 mg $510 现货
418T0 25 mg $1650 现货
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AF 532 NHS ester

NHS-ester of AF 532 dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups.
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目录号 数量 价格 提前通知时间
1C820 1 mg $140 现货
2C820 5 mg $450 现货
4C820 25 mg $1460 现货
5C820 50 mg $1890 现货
6C820 100 mg $2640 现货
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AF 555 NHS ester

AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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目录号 数量 价格 提前通知时间
1K820 1 mg $140 现货
2K820 5 mg $450 现货
4K820 25 mg $1460 现货
5K820 50 mg $1890 现货
6K820 100 mg $2640 现货
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AF 568 NHS ester

AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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目录号 数量 价格 提前通知时间
14820 1 mg $125 现货
24820 5 mg $325 现货
44820 25 mg $720 现货
54820 50 mg $1325 现货
64820 100 mg $2090 现货
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AF 594 NHS酯 (AF 594 NHS ester)

AF 594 染料的红色荧光 NHS-酯,用于标记蛋白质、肽、抗体和任何含有 -NH2 基团的分子。纯5-异构体
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目录号 数量 价格 提前通知时间
2825-1mg 1 mg $125 5 天
2825-5mg 5 mg $425 现货
2825-25mg 25 mg $1240 现货
2825-50mg 50 mg $1890 现货
2825-100mg 100 mg $2640 5 天
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AF 647 NHS 酯 (AF 647 NHS ester)

明亮的水溶性琥珀酰亚胺酯AF 647染料,用于蛋白质和抗体标记。
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目录号 数量 价格 提前通知时间
16820 1 mg $140 现货
26820 5 mg $450 现货
46820 25 mg $1460 现货
56820 50 mg $1890 现货
66820 100 mg $2640 现货
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AMCA NHS ester

NHS-ester of AMCA dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups. AMCA is one of the brightest blue fluorescent dyes with large Stoke's shift and high resistance to photobleaching.
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目录号 数量 价格 提前通知时间
3606-5mg 5 mg $110 现货
3606-25mg 25 mg $290 现货
3606-50mg 50 mg $435 现货
3606-100mg 100 mg $595 现货
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BDP 558/568 NHS ester

BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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目录号 数量 价格 提前通知时间
17420 1 mg $125 现货
27420 5 mg $260 现货
47420 25 mg $510 现货
57420 50 mg $895 现货
67420 100 mg $1490 现货
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BDP 564/570 NHS ester

Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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目录号 数量 价格 提前通知时间
1H420 1 mg $125 现货
2H420 5 mg $325 现货
4H420 25 mg $510 现货
5H420 50 mg $895 现货
6H420 100 mg $1490 现货
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BDP 576/589 NHS 酯 (BDP 576/589 NHS ester)

BDP 576/589 NHS 酯(BODIPY™ 576/589 类似物)是一种明亮的疏水性染料,发射在橙色光谱范围内。它可用于荧光各向异性分析。
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目录号 数量 价格 提前通知时间
1G420 1 mg $125 现货
2G420 5 mg $260 现货
4G420 25 mg $510 现货
5G420 50 mg $895 现货
6G420 100 mg $1490 现货
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BDP 581/591 NHS ester

NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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目录号 数量 价格 提前通知时间
16420 1 mg $125 现货
26420 5 mg $260 现货
46420 25 mg $510 现货
56420 50 mg $895 现货
66420 100 mg $1490 现货
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BDP 630/650-X-NHS 酯 (BDP 630/650-X-NHS ester)

BDP 630/650 是一种硼二吡咯亚甲基荧光团,可调谐至 Cyanine5 激发和发射通道。该染料明亮且光稳定性高。它对于荧光偏振测定很有用。这是一种胺反应性的 NHS 酯衍生物。
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目录号 数量 价格 提前通知时间
15420 1 mg $125 现货
25420 5 mg $260 现货
45420 25 mg $510 现货
55420 50 mg $895 现货
65420 100 mg $1490 现货
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BDP 650/665-X-NHS 酯 (BDP 650/665-X-NHS ester)

BDP 650/665 是一种用于 Cy5 通道的硼二吡咯亚甲基染料,具有胺反应性,具有高摩尔消光系数和良好的发射量子产率。
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目录号 数量 价格 提前通知时间
1E420 1 mg $125 现货
2E420 5 mg $260 现货
4E420 25 mg $510 现货
5E420 50 mg $895 现货
6E420 100 mg $1490 现货