Activated esters

Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.

Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.

NHS ester coupling chemistry

Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.

(5,6)-FAM diacetate NHS ester

Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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2934-1mg 1 mg –   现货
2934-25mg 25 mg 150€ 现货
2934-50mg 50 mg 260€ 现货
2934-100mg 100 mg 510€ 现货
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AF 343 (Coumarin)-X-NHS ester

AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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12620 1 mg 105€ 现货
22620 5 mg 205€ 现货
42620 25 mg 385€ 现货
52620 50 mg 635€ 现货
62620 100 mg 1090€ 现货
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AF 430 NHS ester

AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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10820 1 mg 110€ 现货
20820 5 mg 165€ 现货
40820 25 mg 380€ 现货
50820 50 mg 695€ 现货
60820 100 mg 1190€ 现货
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AF 488 NHS 酯 (AF 488 NHS ester)

AF 488 NHS 酯，一种明亮、光稳定、亲水性的绿色通道荧光染料。
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11820 1 mg 125€ 现货
21820 5 mg 325€ 现货
31820 10 mg 480€ 现货
41820 25 mg 950€ 现货
51820 50 mg 1625€ 现货
61820 100 mg 2090€ 现货
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AF 488 TFP ester

Tetrafluorophenyl ester of green fluorescent dye AF 488 for labeling amino groups in proteins, peptides, and modified oligonucleotides.
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118T0 1 mg 165€ 现货
218T0 5 mg 510€ 现货
418T0 25 mg 1650€ 现货
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AF 532 NHS ester

NHS-ester of AF 532 dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups.
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1C820 1 mg 140€ 现货
2C820 5 mg 450€ 现货
4C820 25 mg 1460€ 现货
5C820 50 mg 1890€ 现货
6C820 100 mg 2640€ 现货
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AF 555 NHS ester

AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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1K820 1 mg 140€ 现货
2K820 5 mg 450€ 现货
4K820 25 mg 1460€ 现货
5K820 50 mg 1890€ 现货
6K820 100 mg 2640€ 现货
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AF 568 NHS ester

AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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14820 1 mg 125€ 现货
24820 5 mg 325€ 现货
44820 25 mg 720€ 现货
54820 50 mg 1325€ 现货
64820 100 mg 2090€ 5 天
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AF 594 NHS酯 (AF 594 NHS ester)

AF 594 染料的红色荧光 NHS-酯，用于标记蛋白质、肽、抗体和任何含有 -NH2 基团的分子。纯5-异构体
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2825-1mg 1 mg 125€ 现货
2825-5mg 5 mg 425€ 现货
2825-25mg 25 mg 1240€ 现货
2825-50mg 50 mg 1890€ 现货
2825-100mg 100 mg 2640€ 现货
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AF 647 NHS 酯 (AF 647 NHS ester)

明亮的水溶性琥珀酰亚胺酯AF 647染料，用于蛋白质和抗体标记。
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16820 1 mg 140€ 现货
26820 5 mg 450€ 现货
46820 25 mg 1460€ 现货
56820 50 mg 1890€ 现货
66820 100 mg 2640€ 现货
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AMCA NHS ester

NHS-ester of AMCA dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups. AMCA is one of the brightest blue fluorescent dyes with large Stoke's shift and high resistance to photobleaching.
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3606-5mg 5 mg 105€ 现货
3606-25mg 25 mg 280€ 现货
3606-50mg 50 mg 430€ 现货
3606-100mg 100 mg 590€ 现货
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BDP 558/568 NHS ester

BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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17420 1 mg 125€ 现货
27420 5 mg 260€ 现货
47420 25 mg 510€ 现货
57420 50 mg 895€ 现货
67420 100 mg 1490€ 现货
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BDP 564/570 NHS ester

Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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1H420 1 mg 125€ 现货
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4H420 25 mg 510€ 现货
5H420 50 mg 895€ 现货
6H420 100 mg 1490€ 现货
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BDP 576/589 NHS 酯 (BDP 576/589 NHS ester)

BDP 576/589 NHS 酯（BODIPY™ 576/589 类似物）是一种明亮的疏水性染料，发射在橙色光谱范围内。它可用于荧光各向异性分析。
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1G420 1 mg 125€ 现货
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5G420 50 mg 895€ 现货
6G420 100 mg 1490€ 现货
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BDP 581/591 NHS ester

NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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16420 1 mg 125€ 现货
26420 5 mg 260€ 现货
46420 25 mg 510€ 现货
56420 50 mg 895€ 现货
66420 100 mg 1490€ 现货
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BDP 630/650-X-NHS 酯 (BDP 630/650-X-NHS ester)

BDP 630/650 是一种硼二吡咯亚甲基荧光团，可调谐至 Cyanine5 激发和发射通道。该染料明亮且光稳定性高。它对于荧光偏振测定很有用。这是一种胺反应性的 NHS 酯衍生物。
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15420 1 mg 125€ 现货
25420 5 mg 260€ 现货
45420 25 mg 510€ 现货
55420 50 mg 895€ 现货
65420 100 mg 1490€ 现货
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BDP 650/665-X-NHS 酯 (BDP 650/665-X-NHS ester)

BDP 650/665 是一种用于 Cy5 通道的硼二吡咯亚甲基染料，具有胺反应性，具有高摩尔消光系数和良好的发射量子产率。
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1E420 1 mg 125€ 现货
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4E420 25 mg 510€ 现货
5E420 50 mg 895€ 现货
6E420 100 mg 1490€ 现货
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BDPep FL NHS ester

BDP FL analog derivative suitable for solid phase synthesis SPPS.
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1J420 1 mg –   现货
2J420 5 mg 260€ 现货
4J420 25 mg 510€ 现货
5J420 50 mg 895€ 现货
6J420 100 mg 1490€ 现货
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BDP FL NHS 酯 (BDP FL NHS ester)

氨基反应性 BDP FL，一种非常明亮且光稳定的荧光素通道染料。
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11420 1 mg 125€ 98€ 现货
21420 5 mg 260€ 175€ 现货
41420 25 mg 485€ 现货
51420 50 mg 824€ 现货
61420 100 mg 1380€ 现货
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BDP R6G NHS ester

BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
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14420 1 mg 125€ 现货
24420 5 mg 260€ 现货
44420 25 mg 510€ 现货
54420 50 mg 895€ 现货
64420 100 mg 1490€ 现货
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BDP TMR NHS 酯

BDP TMR 的胺基反应活性 NHS 酯，明亮的硼二吡咯亚甲基染料，可用于 TAMRA 通道，特别适用于荧光偏振测定。
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12420 1 mg 125€ 现货
22420 5 mg 260€ 现货
42420 25 mg 510€ 现货
52420 50 mg 895€ 现货
62420 100 mg 1490€ 现货
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BDP TMR-X-NHS ester

Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays. This compound contains an aminohexanoic acid (C6) linker between the dye and the functional group.
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1F420 1 mg 125€ 现货
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4F420 25 mg 510€ 现货
5F420 50 mg 895€ 现货
6F420 100 mg 1490€ 现货
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BDP TR-X-NHS 酯 (BDP TR-X-NHS ester)

BDP TR-X-NHS 酯是 BDP TR 的衍生物，含有基于氨基己酸 (C6) 的长连接体。该染料的吸收和发射接近ROX染料。NHS 酯可用于偶合蛋白质和肽。
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1D420 1 mg 125€ 现货
2D420 5 mg 325€ 现货
4D420 25 mg 510€ 现货
5D420 50 mg 895€ 现货
6D420 100 mg 1490€ 现货
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Cyanine3.5 NHS ester (Cyanine3.5 NHS酯)

Cyanine3.5染料NHS酯的衍生物。
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12020 1 mg 125€ 现货
22020 5 mg 260€ 现货
42020 25 mg 510€ 现货
52020 50 mg 895€ 现货
62020 100 mg 1490€ 现货
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Cyanine3B NHS 酯 (Cyanine3B NHS ester)

Cyanine3B NHS 酯是一种活性染料，可显著提高荧光量子产率和光稳定性，用于生物分子中氨基的特异性标记。
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19320 1 mg 140€ 现货
29320 5 mg 340€ 现货
49320 25 mg 990€ 现货
59320 50 mg 1690€ 现货
69320 100 mg 3100€ 现货
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Cyanine3 NHS酯

胺反应性 Cyanine3 染料 NHS 酯。
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11020 1 mg 91€ 现货
21020 5 mg 119€ 现货
41020 25 mg 380€ 现货
51020 50 mg 670€ 现货
61020 100 mg 1140€ 现货
81020 1 g 请查询 12 天
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Cyanine5.5 NHS酯 (Cyanine5.5 NHS ester)

Cyanine5.5 NHS 是一种远红/近红外胺反应性染料。
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17020 1 mg 125€ 现货
27020 5 mg 260€ 现货
47020 25 mg 510€ 现货
57020 50 mg 895€ 现货
67020 100 mg 1490€ 现货
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Cyanine5 NHS 酯 (Cyanine5 NHS ester)

胺反应性 Cyanine5 活化酯，用于标记蛋白质、肽和其他分子。
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13020 1 mg 87€ 现货
23020 5 mg 117€ 现货
43020 25 mg 337€ 现货
53020 50 mg 550€ 现货
63020 100 mg 870€ 现货
83020 1 g 请查询 12 天
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Cyanine7.5 NHS酯 (Cyanine7.5 NHS ester)

近红外荧光染料 Cyanine7.5 的胺反应性的 NHS 酯。
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16020 1 mg 125€ 现货
26020 5 mg 260€ 现货
46020 25 mg 510€ 现货
56020 50 mg 895€ 现货
66020 100 mg 1490€ 现货
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Cyanine7 NHS 酯 (Cyanine7 NHS ester)

近红外染料 Cyanine7 的胺反应活性 NHS 酯。
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15020 1 mg 125€ 现货
25020 5 mg 260€ 5 天
45020 25 mg 510€ 现货
55020 50 mg 895€ 现货
65020 100 mg 1490€ 现货
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DusQ 1 NHS ester

Functionalized DusQ 1 quencher derivative with quenching range from 480 - 580 nm for labeling of amine-containing molecules.
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21D20 5 mg –   现货
41D20 25 mg 310€ 现货
51D20 50 mg 575€ 现货
61D20 100 mg 880€ 现货
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DusQ 21 NHS ester

Amino-reactive NHS ester with quencher for the conjugation with amines-containing molecules, DusQ 21 quenching range 590 - 720 nm.
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28D20 5 mg –   现货
48D20 25 mg 310€ 现货
68D20 100 mg 880€ 现货
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DusQ 2 NHS ester

DusQ 2 dark quencher with a quenching range from 560 - 670 nm with primary amine reactivity for synthesis of FRET probes. This NHS ester can be coupled to various molecules bearing amino groups.
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22D20 5 mg –   现货
42D20 25 mg 310€ 现货
52D20 50 mg 575€ 现货
62D20 100 mg 880€ 现货
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DusQ 3 NHS ester

Amino-reactive NHS ester for the labeling with far-red quencher DusQ 3.
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23D20 5 mg –   现货
43D20 25 mg 310€ 现货
53D20 50 mg 575€ 现货
63D20 100 mg 880€ 现货
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FAM isothiocyanate (FITC), 5- and 6-isomers

An isothiocyanate derivative of fluorescein for labeling of antibodies and other biomolecules. Mix of 5- and 6-isomers.
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3524-100mg 100 mg –   现货
3524-500mg 500 mg 239€ 现货
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FAM NHS ester, 6-isomer

Activated N-hydroxysuccinimide ester of fluorescein (FAM), pure 6-isomer.
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15120 1 mg –   现货
25120 5 mg 105€ 现货
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HEX NHS ester, 6-isomer

NHS-ester of HEX dye for proteins, peptides, and modified oligonucleotides labeling via reaction with amino groups.
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2B220 5 mg 165€ 现货
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JOE NHS ester, 5-isomer

JOE is a fluorescent dye with emission in the yellow part of the spectrum. This derivative is an activated NHS ester for conjugation with amines.
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11120 1 mg 105€ 现货
21120 5 mg 205€ 现货
41120 25 mg 385€ 现货
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Pyrenebutyric acid NHS ester

Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.
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63520 100 mg 105€ 现货
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R6G NHS ester, 5-isomer

R6G (rhodamine 6G) NHS ester, pure 5-isomer. This dye is often used for the labeling of oligonucleotides for qPCR.
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14220 1 mg 105€ 现货
24220 5 mg 205€ 现货
44220 25 mg 385€ 现货
54220 50 mg 635€ 现货
64220 100 mg 1090€ 现货
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ROX NHS ester, 5-isomer

Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.
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11220 1 mg 105€ 现货
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41220 25 mg 385€ 现货
51220 50 mg 635€ 现货
61220 100 mg 1090€ 现货
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ROX NHS 酯，6-异构体

ROX（罗丹明X，R101）NHS-酯的纯 6-异构体，用于胺标记。
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12220 1 mg 105€ 现货
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42220 25 mg 385€ 现货
52220 50 mg 635€ 5 天
62220 100 mg 1090€ 5 天
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sulfo-Cyanine3.5 NHS ester

Amine-reactive activated ester of water-soluble, sulfonated sulfo-Cyanine3.5 dye.
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12320 1 mg 125€ 现货
22320 5 mg 325€ 现货
42320 25 mg 850€ 现货
52320 50 mg 1490€ 现货
62320 100 mg 1990€ 现货
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sulfo-Cyanine3 NHS酯 (sulfo-Cyanine3 NHS ester)

水溶性磺基-Cyanine3琥珀酰亚胺酯 (SE) 活化酯，用于氨基生物分子标记。
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11320 1 mg 91€ 现货
21320 5 mg 119€ 现货
41320 25 mg 395€ 现货
51320 50 mg 729€ 现货
61320 100 mg 1290€ 现货
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磺基-Cyanine5.5 NHS酯 (sulfo-Cyanine5.5 NHS ester)

水溶性、磺化、远红外磺基-Cyanine5.5 染料，以NHS 酯的形式存在。
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17320 1 mg 125€ 现货
27320 5 mg 325€ 现货
47320 25 mg 850€ 现货
57320 50 mg 1490€ 现货
67320 100 mg 1990€ 现货
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sulfo-Cyanine5 bis-NHS ester

Water soluble amine reactive Cyanine5 dye with two NHS ester groups.
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133D0 1 mg 125€ 现货
233D0 5 mg 325€ 现货
433D0 25 mg 850€ 现货
533D0 50 mg 1490€ 现货
633D0 100 mg 1990€ 现货
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磺基-Cyanine5 NHS 酯 (sulfo-Cyanine5 NHS ester)

水溶性磺基-Cyanine5 琥珀酰亚胺酯，用于在水相中标记各种含胺分子。
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13320 1 mg 91€ 现货
23320 5 mg 119€ 现货
43320 25 mg 345€ 现货
53320 50 mg 557€ 现货
63320 100 mg 890€ 现货
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磺基-Cyanine7.5 NHS酯 (sulfo-Cyanine7.5 NHS ester)

一种用于活体成像的水溶性近红外发射染料。 sulfo-Cyanine7.5 是吲哚菁绿 (ICG) 的类似物，具有较高的发射量子产率和连接臂。
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目录号 数量 价格 提前通知时间
16320 1 mg 125€ 8 天
26320 5 mg 325€ 5 天
46320 25 mg 850€ 现货
56320 50 mg 1490€ 现货
66320 100 mg 1990€ 现货
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sulfo-Cyanine7 bis-NHS ester

sulfo-Cyanine7 bis-NHS ester is a bifunctional derivative of water-soluble near-infrared sulfo-Cyanine7. It contains two activated ester functional groups.
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目录号 数量 价格 提前通知时间
153D0 1 mg 125€ 现货
253D0 5 mg 325€ 现货
453D0 25 mg 850€ 现货
553D0 50 mg 1490€ 现货
653D0 100 mg 1990€ 现货
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磺基-Cyanine7 NHS 酯 (sulfo-Cyanine7 NHS ester)

sulfo-Cyanine7 NHS 酯是一种水溶性、近红外、胺反应性染料。
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目录号 数量 价格 提前通知时间
15320 1 mg 125€ 现货
25320 5 mg 325€ 现货
45320 25 mg 850€ 现货
55320 50 mg 1490€ 现货
65320 100 mg 1880€ 现货
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TAMRA NHS 酯，5-异构体 (TAMRA NHS ester, 5-isomer)

胺反应性、活化的 TAMRA 染料 N-羟基琥珀酰亚胺酯，用于标记蛋白质、多肽和氨基寡核苷酸的胺基。纯5-异构体。
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目录号 数量 价格 提前通知时间
17120 1 mg –   现货
27120 5 mg 210€ 现货
47120 25 mg 345€ 现货
57120 50 mg 595€ 现货
67120 100 mg 790€ 现货
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TAMRA NHS ester, 6-isomer

NHS-ester of TAMRA dye for proteins, peptides, and modified oligonucleotides labeling via reaction with amino groups. Pure 6-isomer.
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目录号 数量 价格 提前通知时间
18120 1 mg 105€ 现货
28120 5 mg 205€ 现货
48120 25 mg 345€ 现货
58120 50 mg 595€ 5 天
68120 100 mg 790€ 现货
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Thiazole Orange NHS ester

Thiazole Orange NHS ester for labeling biomolecules (peptides, proteins, amino-DNA, etc.). Thiazole orange is a cyanine dye that is essentially dark in solution but has a bright fluorescence in double-stranded DNA and RNA.
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目录号 数量 价格 提前通知时间
2183-1mg 1 mg –   现货
2183-5mg 5 mg 60€ 现货
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TR-X-NHS 酯，5-异构体 (TR-X-NHS ester, 5-isomer)

TR（又名德克萨斯红）是一种基于磺基罗丹明骨架的红色荧光染料，是一种胺反应性的NHS 酯。
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目录号 数量 价格 提前通知时间
1V220 1 mg 125€ 现货
2V220 5 mg 325€ 现货
4V220 25 mg 510€ 现货
5V220 50 mg 895€ 5 天
6V220 100 mg 1490€ 现货
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