活化酯

Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.

Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.

NHS ester coupling chemistry

Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.

(5,6)-FAM diacetate NHS ester

Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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目录号 数量 价格 提前通知时间
2934-1mg 1 mg –   现货
2934-25mg 25 mg $160 现货
2934-50mg 50 mg $270 现货
2934-100mg 100 mg $520 现货
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AF 343(香豆素)-X-NHS 酯 (AF 343 (Coumarin)-X-NHS ester)

AF 343(香豆素)是一种蓝光发射染料,可用作荧光素 (FAM) 的 FRET 供体。该结构的特点是香豆素 343 荧光团和官能团之间有一个连接体。它提供了更好的溶解性以及荧光团和官能团之间的空间分离。
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目录号 数量 价格 提前通知时间
12620 1 mg $110 现货
22620 5 mg $210 现货
42620 25 mg $410 现货
52620 50 mg $695 现货
62620 100 mg $1190 现货
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AF 430 NHS ester

AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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目录号 数量 价格 提前通知时间
10820 1 mg $110 现货
20820 5 mg $165 现货
40820 25 mg $380 现货
50820 50 mg $695 现货
60820 100 mg $1190 现货
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AF 488 NHS 酯 (AF 488 NHS ester)

AF 488 NHS 酯,一种明亮、光稳定、亲水性的绿色通道荧光染料。
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目录号 数量 价格 提前通知时间
11820 1 mg $125 现货
21820 5 mg $325 现货
31820 10 mg $480 现货
41820 25 mg $950 现货
51820 50 mg $1625 现货
61820 100 mg $2090 现货
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AF 488 TFP 酯 (AF 488 TFP ester)

绿色荧光染料 AF 488 的四氟苯酯,用于标记蛋白质、多肽和修饰寡核苷酸中的氨基。
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目录号 数量 价格 提前通知时间
118T0 1 mg $165 21 天
218T0 5 mg $510 现货
418T0 25 mg $1650 现货
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AF 532 NHS 酯 (AF 532 NHS ester)

AF 532 染料的 NHS 酯,通过与氨基反应标记蛋白质、多肽和修饰寡核苷酸。
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目录号 数量 价格 提前通知时间
1C820 1 mg $140 现货
2C820 5 mg $450 现货
4C820 25 mg $1460 现货
5C820 50 mg $1890 现货
6C820 100 mg $2640 现货
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AF 555 NHS ester

AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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目录号 数量 价格 提前通知时间
1K820 1 mg $140 现货
2K820 5 mg $450 现货
4K820 25 mg $1460 现货
5K820 50 mg $1890 现货
6K820 100 mg $2640 现货
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AF 568 NHS ester

AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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目录号 数量 价格 提前通知时间
14820 1 mg $125 现货
24820 5 mg $325 现货
44820 25 mg $720 现货
54820 50 mg $1325 现货
64820 100 mg $2090 现货
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AF 594 NHS酯 (AF 594 NHS ester)

AF 594 染料的红色荧光 NHS-酯,用于标记蛋白质、肽、抗体和任何含有 -NH2 基团的分子。纯5-异构体
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目录号 数量 价格 提前通知时间
2825-1mg 1 mg $125 现货
2825-5mg 5 mg $425 现货
2825-25mg 25 mg $1240 现货
2825-50mg 50 mg $1890 现货
2825-100mg 100 mg $2640 现货
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AF 647 NHS 酯 (AF 647 NHS ester)

明亮的水溶性琥珀酰亚胺酯AF 647染料,用于蛋白质和抗体标记。
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目录号 数量 价格 提前通知时间
16820 1 mg $140 5 天
26820 5 mg $450 7 天
46820 25 mg $1460 现货
56820 50 mg $1890 现货
66820 100 mg $2640 现货
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AMCA NHS ester

NHS-ester of AMCA dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups. AMCA is one of the brightest blue fluorescent dyes with large Stoke's shift and high resistance to photobleaching.
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目录号 数量 价格 提前通知时间
3606-5mg 5 mg $110 现货
3606-25mg 25 mg $290 现货
3606-50mg 50 mg $435 现货
3606-100mg 100 mg $595 现货
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ATT 550 NHS 酯 (ATT 550 NHS ester)

水溶性 ATT 550 琥珀酰亚胺酯,用于标记水相中的各种含胺分子。
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目录号 数量 价格 提前通知时间
1C920 1 mg $110 现货
2C920 5 mg $640 现货
4C920 25 mg $1640 现货
5C920 50 mg $2090 现货
6C920 100 mg $2840 现货
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BDP® 558/568 NHS ester

BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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目录号 数量 价格 提前通知时间
17420 1 mg $125 现货
27420 5 mg $260 现货
47420 25 mg $510 现货
57420 50 mg $895 现货
67420 100 mg $1490 现货
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BDP 564/570 NHS ester

Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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目录号 数量 价格 提前通知时间
1H420 1 mg $125 现货
2H420 5 mg $325 现货
4H420 25 mg $510 现货
5H420 50 mg $895 现货
6H420 100 mg $1490 现货
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BDP® 576/589 NHS 酯 (BDP® 576/589 NHS ester)

BDP 576/589 NHS 酯(BODIPY™ 576/589 类似物)是一种明亮的疏水性染料,发射在橙色光谱范围内。它可用于荧光各向异性分析。
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目录号 数量 价格 提前通知时间
1G420 1 mg $125 现货
2G420 5 mg $260 现货
4G420 25 mg $510 现货
5G420 50 mg $895 现货
6G420 100 mg $1490 现货
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BDP® 581/591 NHS ester

NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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目录号 数量 价格 提前通知时间
16420 1 mg $125 现货
26420 5 mg $260 现货
46420 25 mg $510 现货
56420 50 mg $895 现货
66420 100 mg $1490 现货
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BDP® 630/650-X-NHS 酯 (BDP® 630/650-X-NHS ester)

BDP 630/650 是一种硼二吡咯亚甲基荧光团,可调谐至 Cyanine5 激发和发射通道。该染料明亮且光稳定性高。它对于荧光偏振测定很有用。这是一种胺反应性的 NHS 酯衍生物。
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目录号 数量 价格 提前通知时间
15420 1 mg $125 现货
25420 5 mg $260 现货
45420 25 mg $510 现货
55420 50 mg $895 现货
65420 100 mg $1490 现货
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BDP® 650/665-X-NHS 酯 (BDP® 650/665-X-NHS ester)

BDP 650/665 是一种用于 Cy5 通道的硼二吡咯亚甲基染料,具有胺反应性,具有高摩尔消光系数和良好的发射量子产率。
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目录号 数量 价格 提前通知时间
1E420 1 mg $125 现货
2E420 5 mg $260 现货
4E420 25 mg $510 现货
5E420 50 mg $895 现货
6E420 100 mg $1490 现货
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BDPep 630/650 NHS ester

Dicyano BDP 630/650 is a far-red fluorescent dye from the boron dipyrromethene (BDP) family, known for its high brightness and exceptional photostability. Its remarkable stability in strong acidic TFA media makes it particularly suitable for solid-phase peptide synthesis (SPPS). BDPep 630/650 NHS ester can be used for effective cell sorting and phenotyping.
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目录号 数量 价格 提前通知时间
1M420 1 mg $125 现货
2M420 5 mg $260 现货
4M420 25 mg $510 现货
5M420 50 mg $895 21 天
6M420 100 mg $1490 21 天
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BDPep FL NHS ester

BDP FL analog derivative suitable for solid phase synthesis SPPS.
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目录号 数量 价格 提前通知时间
1J420 1 mg –   现货
2J420 5 mg $260 现货
4J420 25 mg $510 现货
5J420 50 mg $895 现货
6J420 100 mg $1490 现货
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BDP® FL NHS 酯 (BDP® FL NHS ester)

氨基反应性 BDP FL,一种非常明亮且光稳定的荧光素通道染料。
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目录号 数量 价格 提前通知时间
11420 1 mg $125 $98 现货
21420 5 mg $260 $175 现货
41420 25 mg $485 现货
51420 50 mg $824 现货
61420 100 mg $1380 现货
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BDP® R6G NHS 酯 (BDP® R6G NHS ester)

BDP R6G 是一种硼二吡咯亚甲基染料,其吸收光谱和发射光谱与 R6G 相似。
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目录号 数量 价格 提前通知时间
14420 1 mg $125 现货
24420 5 mg $260 现货
44420 25 mg $510 现货
54420 50 mg $895 现货
64420 100 mg $1490 现货
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BDP® TMR NHS 酯

BDP TMR 的胺基反应活性 NHS 酯,明亮的硼二吡咯亚甲基染料,可用于 TAMRA 通道,特别适用于荧光偏振测定。
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目录号 数量 价格 提前通知时间
12420 1 mg $125 现货
22420 5 mg $260 现货
42420 25 mg $510 现货
52420 50 mg $895 现货
62420 100 mg $1490 现货
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BDP® TMR-X-NHS ester

Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays. This compound contains an aminohexanoic acid (C6) linker between the dye and the functional group.
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目录号 数量 价格 提前通知时间
1F420 1 mg $125 现货
2F420 5 mg $260 现货
4F420 25 mg $510 现货
5F420 50 mg $895 现货
6F420 100 mg $1490 现货
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BDP® TR-X-NHS 酯 (BDP® TR-X-NHS ester)

BDP TR-X-NHS 酯是 BDP TR 的衍生物,含有基于氨基己酸 (C6) 的长连接体。该染料的吸收和发射接近ROX染料。NHS 酯可用于偶合蛋白质和肽。
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目录号 数量 价格 提前通知时间
1D420 1 mg $125 现货
2D420 5 mg $325 现货
4D420 25 mg $510 现货
5D420 50 mg $895 现货
6D420 100 mg $1490 现货
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Cyanine3.5 NHS ester (Cyanine3.5 NHS酯)

Cyanine3.5染料NHS酯的衍生物。
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目录号 数量 价格 提前通知时间
12020 1 mg $125 现货
22020 5 mg $260 现货
42020 25 mg $510 现货
52020 50 mg $895 现货
62020 100 mg $1490 现货
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Cyanine3B NHS 酯 (Cyanine3B NHS ester)

Cyanine3B NHS 酯是一种活性染料,可显著提高荧光量子产率和光稳定性,用于生物分子中氨基的特异性标记。
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目录号 数量 价格 提前通知时间
19320 1 mg $140 现货
29320 5 mg $340 现货
49320 25 mg $990 现货
59320 50 mg $1690 现货
69320 100 mg $3100 现货
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Cyanine3 NHS酯

胺反应性 Cyanine3 染料 NHS 酯。
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目录号 数量 价格 提前通知时间
11020 1 mg $91 现货
21020 5 mg $119 现货
41020 25 mg $380 现货
51020 50 mg $670 现货
61020 100 mg $1150 现货
81020 1 g 请查询 现货
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Cyanine5.5 NHS酯 (Cyanine5.5 NHS ester)

Cyanine5.5 NHS 是一种远红/近红外胺反应性染料。
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目录号 数量 价格 提前通知时间
17020 1 mg $125 现货
27020 5 mg $260 现货
47020 25 mg $510 现货
57020 50 mg $895 现货
67020 100 mg $1490 现货
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Cyanine5 NHS 酯 (Cyanine5 NHS ester)

胺反应性 Cyanine5 活化酯,用于标记蛋白质、肽和其他分子。
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目录号 数量 价格 提前通知时间
13020 1 mg $87 现货
23020 5 mg $117 现货
43020 25 mg $337 现货
53020 50 mg $550 现货
63020 100 mg $870 现货
83020 1 g 请查询 12 天
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Cyanine7.5 NHS酯 (Cyanine7.5 NHS ester)

近红外荧光染料 Cyanine7.5 的胺反应性的 NHS 酯。
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目录号 数量 价格 提前通知时间
16020 1 mg $125 现货
26020 5 mg $260 现货
46020 25 mg $510 现货
56020 50 mg $895 现货
66020 100 mg $1490 现货
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Cyanine7 NHS 酯 (Cyanine7 NHS ester)

近红外染料 Cyanine7 的胺反应活性 NHS 酯。
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目录号 数量 价格 提前通知时间
15020 1 mg $125 现货
25020 5 mg $260 现货
45020 25 mg $510 现货
55020 50 mg $895 现货
65020 100 mg $1490 现货
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DusQ 1 NHS ester

Functionalized DusQ 1 quencher derivative with quenching range from 480 - 580 nm for labeling of amine-containing molecules.
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21D20 5 mg –   现货
41D20 25 mg $315 现货
51D20 50 mg $580 现货
61D20 100 mg $885 现货
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DusQ 21 NHS ester

Amino-reactive NHS ester with quencher for the conjugation with amines-containing molecules, DusQ 21 quenching range 590 - 720 nm.
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目录号 数量 价格 提前通知时间
28D20 5 mg –   现货
48D20 25 mg $315 现货
68D20 100 mg $885 现货
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DusQ 2 NHS 酯 (DusQ 2 NHS ester)

DusQ 2 暗淬灭剂,淬灭范围为 560 - 670 nm,具有伯胺反应活性,用于合成 FRET 探针。这种 NHS 酯可以与各种带有氨基的分子偶联。
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目录号 数量 价格 提前通知时间
22D20 5 mg –   现货
42D20 25 mg $315 现货
52D20 50 mg $580 现货
62D20 100 mg $885 现货
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DusQ 3 NHS ester

Amino-reactive NHS ester for the labeling with far-red quencher DusQ 3.
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目录号 数量 价格 提前通知时间
23D20 5 mg –   现货
43D20 25 mg $315 现货
53D20 50 mg $580 现货
63D20 100 mg $885 现货
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FAM isothiocyanate (FITC), 5- and 6-isomers

An isothiocyanate derivative of fluorescein for labeling of antibodies and other biomolecules. Mix of 5- and 6-isomers.
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3524-100mg 100 mg $45 现货
3524-500mg 500 mg $249 $149 现货
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FAM NHS 酯,6-异构体 (FAM NHS ester, 6-isomer)

活化的荧光素 N-羟基琥珀酰亚胺酯 (FAM),纯 6-异构体。
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目录号 数量 价格 提前通知时间
15120 1 mg –   现货
25120 5 mg $110 现货
45120 25 mg $145 现货
55120 50 mg $260 现货
65120 100 mg $470 现货
85120 1 g 请查询 现货
95120 5 g 请查询 现货
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HEX NHS ester, 6-isomer

NHS-ester of HEX dye for proteins, peptides, and modified oligonucleotides labeling via reaction with amino groups.
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2B220 5 mg $175 现货
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JOE NHS ester, 5-isomer

JOE is a fluorescent dye with emission in the yellow part of the spectrum. This derivative is an activated NHS ester for conjugation with amines.
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目录号 数量 价格 提前通知时间
11120 1 mg $110 现货
21120 5 mg $210 现货
41120 25 mg $410 现货
51120 50 mg $695 现货
61120 100 mg $1190 现货
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芘丁酸NHS酯 (Pyrenebutyric acid NHS ester)

芘 NHS 酯,用于用芘标记生物分子。芘是一种可形成激发物的荧光探针,也是固定石墨的锚定基团。
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63520 100 mg $110 现货
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R6G NHS ester, 5-isomer

R6G (rhodamine 6G) NHS ester, pure 5-isomer. This dye is often used for the labeling of oligonucleotides for qPCR.
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目录号 数量 价格 提前通知时间
14220 1 mg $110 现货
24220 5 mg $210 现货
44220 25 mg $410 现货
54220 50 mg $695 现货
64220 100 mg $1190 现货
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ROX NHS ester, 5-isomer

Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.
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目录号 数量 价格 提前通知时间
11220 1 mg $110 现货
21220 5 mg $210 现货
41220 25 mg $410 现货
51220 50 mg $695 现货
61220 100 mg $1190 现货
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ROX NHS 酯,6-异构体

ROX(罗丹明X,R101)NHS-酯的纯 6-异构体,用于胺标记。
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目录号 数量 价格 提前通知时间
12220 1 mg $110 现货
22220 5 mg $210 5 天
42220 25 mg $410 现货
52220 50 mg $695 现货
62220 100 mg $1190 现货
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sulfo-Cyanine3.5 NHS ester

Amine-reactive activated ester of water-soluble, sulfonated sulfo-Cyanine3.5 dye.
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目录号 数量 价格 提前通知时间
12320 1 mg $125 现货
22320 5 mg $325 现货
42320 25 mg $850 现货
52320 50 mg $1490 现货
62320 100 mg $1990 现货
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sulfo-Cyanine3 NHS酯 (sulfo-Cyanine3 NHS ester)

水溶性磺基-Cyanine3琥珀酰亚胺酯 (SE) 活化酯,用于氨基生物分子标记。
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目录号 数量 价格 提前通知时间
11320 1 mg $91 现货
21320 5 mg $119 现货
41320 25 mg $395 现货
51320 50 mg $729 现货
61320 100 mg $1290 现货
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磺基-Cyanine5.5 NHS酯 (sulfo-Cyanine5.5 NHS ester)

水溶性、磺化、远红外磺基-Cyanine5.5 染料,以NHS 酯的形式存在。
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目录号 数量 价格 提前通知时间
17320 1 mg $125 5 天
27320 5 mg $325 现货
47320 25 mg $850 现货
57320 50 mg $1490 现货
67320 100 mg $1990 5 天
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sulfo-Cyanine5 alkyne NHS ester

Bifunctional derivative of sulfo-Cyanine5 dye containing reactive groups of terminal alkyne and NHS ester.
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磺基-Cyanine5 双-NHS 酯 (sulfo-Cyanine5 bis-NHS ester)

具有两个 NHS 酯基团的水溶性胺反应性 Cyanine5 染料。
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目录号 数量 价格 提前通知时间
133D0 1 mg $125 现货
233D0 5 mg $325 现货
433D0 25 mg $850 现货
533D0 50 mg $1490 现货
633D0 100 mg $1990 现货
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磺基-Cyanine5 NHS 酯 (sulfo-Cyanine5 NHS ester)

水溶性磺基-Cyanine5 琥珀酰亚胺酯,用于在水相中标记各种含胺分子。
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目录号 数量 价格 提前通知时间
13320 1 mg $91 现货
23320 5 mg $119 现货
43320 25 mg $345 现货
53320 50 mg $557 现货
63320 100 mg $890 现货
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磺基-Cyanine7.5 NHS酯 (sulfo-Cyanine7.5 NHS ester)

一种用于活体成像的水溶性近红外发射染料。 sulfo-Cyanine7.5 是吲哚菁绿 (ICG) 的类似物,具有较高的发射量子产率和连接臂。
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目录号 数量 价格 提前通知时间
16320 1 mg $125 现货
26320 5 mg $325 现货
46320 25 mg $850 现货
56320 50 mg $1490 现货
66320 100 mg $1990 现货
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sulfo-Cyanine7 bis-NHS ester

sulfo-Cyanine7 bis-NHS ester is a bifunctional derivative of water-soluble near-infrared sulfo-Cyanine7. It contains two activated ester functional groups.
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目录号 数量 价格 提前通知时间
153D0 1 mg $125 现货
253D0 5 mg $325 现货
453D0 25 mg $850 现货
553D0 50 mg $1490 现货
653D0 100 mg $1990 现货
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磺基-Cyanine7 NHS 酯 (sulfo-Cyanine7 NHS ester)

sulfo-Cyanine7 NHS 酯是一种水溶性、近红外、胺反应性染料。
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目录号 数量 价格 提前通知时间
15320 1 mg $125 现货
25320 5 mg $325 现货
45320 25 mg $850 现货
55320 50 mg $1490 现货
65320 100 mg $1990 现货
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TAMRA NHS 酯,5-异构体 (TAMRA NHS ester, 5-isomer)

胺反应性、活化的 TAMRA 染料 N-羟基琥珀酰亚胺酯,用于标记蛋白质、多肽和氨基寡核苷酸的胺基。纯5-异构体。
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目录号 数量 价格 提前通知时间
17120 1 mg –   现货
27120 5 mg $210 现货
47120 25 mg $350 现货
57120 50 mg $595 现货
67120 100 mg $790 现货
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TAMRA NHS 酯,6-异构体 (TAMRA NHS ester, 6-isomer)

TAMRA 染料的 NHS-酯,通过与氨基反应标记蛋白质、多肽和修饰寡核苷酸。纯6-异构体。
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目录号 数量 价格 提前通知时间
18120 1 mg $110 现货
28120 5 mg $210 现货
48120 25 mg $350 现货
58120 50 mg $595 现货
68120 100 mg $790 5 天
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Thiazole Orange NHS ester

Thiazole Orange NHS ester for labeling biomolecules (peptides, proteins, amino-DNA, etc.). Thiazole orange is a cyanine dye that is essentially dark in solution but has a bright fluorescence in double-stranded DNA and RNA.
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2183-1mg 1 mg –   现货
2183-5mg 5 mg $60 现货
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TR-X-NHS 酯,5-异构体 (TR-X-NHS ester, 5-isomer)

TR(又名德克萨斯红)是一种基于磺基罗丹明骨架的红色荧光染料,是一种胺反应性的NHS 酯。
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目录号 数量 价格 提前通知时间
1V220 1 mg $125 现货
2V220 5 mg $325 现货
4V220 25 mg $510 现货
5V220 50 mg $895 现货
6V220 100 mg $1490 现货
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