活化酯
Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.
Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.
Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.
(5,6)-FAM diacetate NHS ester
Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2934-1mg | 1 mg | – | 现货 | |
| 2934-25mg | 25 mg | $160 | 现货 | |
| 2934-50mg | 50 mg | $270 | 现货 | |
| 2934-100mg | 100 mg |
$520
|
现货 |
AF 343（香豆素）-X-NHS 酯 (AF 343 (Coumarin)-X-NHS ester)
AF 343（香豆素）是一种蓝光发射染料，可用作荧光素 (FAM) 的 FRET 供体。该结构的特点是香豆素 343 荧光团和官能团之间有一个连接体。它提供了更好的溶解性以及荧光团和官能团之间的空间分离。| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 12620 | 1 mg | $110 | 现货 | |
| 22620 | 5 mg | $210 | 现货 | |
| 42620 | 25 mg |
$410
|
现货 | |
| 52620 | 50 mg |
$695
|
现货 | |
| 62620 | 100 mg |
$1190
|
现货 |
AF 430 NHS ester
AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 10820 | 1 mg | $110 | 现货 | |
| 20820 | 5 mg | $165 | 现货 | |
| 40820 | 25 mg | $380 | 现货 | |
| 50820 | 50 mg |
$695
|
现货 | |
| 60820 | 100 mg |
$1190
|
现货 |
AF 488 NHS 酯 (AF 488 NHS ester)
AF 488 NHS 酯，一种明亮、光稳定、亲水性的绿色通道荧光染料。| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 11820 | 1 mg | $125 | 现货 | |
| 21820 | 5 mg | $325 | 现货 | |
| 31820 | 10 mg |
$480
|
现货 | |
| 41820 | 25 mg |
$950
|
现货 | |
| 51820 | 50 mg |
$1625
|
现货 | |
| 61820 | 100 mg |
$2090
|
现货 |
AF 488 TFP 酯 (AF 488 TFP ester)
绿色荧光染料 AF 488 的四氟苯酯，用于标记蛋白质、多肽和修饰寡核苷酸中的氨基。| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 118T0 | 1 mg | $165 | 现货 | |
| 218T0 | 5 mg |
$510
|
现货 | |
| 418T0 | 25 mg |
$1650
|
现货 |
AF 532 NHS 酯 (AF 532 NHS ester)
AF 532 染料的 NHS 酯，通过与氨基反应标记蛋白质、多肽和修饰寡核苷酸。| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 1C820 | 1 mg | $140 | 现货 | |
| 2C820 | 5 mg |
$450
|
现货 | |
| 4C820 | 25 mg |
$1460
|
现货 | |
| 5C820 | 50 mg |
$1890
|
现货 | |
| 6C820 | 100 mg |
$2640
|
现货 |
AF 555 NHS ester
AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 1K820 | 1 mg | $140 | 现货 | |
| 2K820 | 5 mg |
$450
|
现货 | |
| 4K820 | 25 mg |
$1460
|
现货 | |
| 5K820 | 50 mg |
$1890
|
现货 | |
| 6K820 | 100 mg |
$2640
|
现货 |
AF 568 NHS ester
AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 14820 | 1 mg | $125 | 现货 | |
| 24820 | 5 mg | $325 | 现货 | |
| 44820 | 25 mg |
$720
|
现货 | |
| 54820 | 50 mg |
$1325
|
现货 | |
| 64820 | 100 mg |
$2090
|
现货 |
AF 594 NHS酯 (AF 594 NHS ester)
AF 594 染料的红色荧光 NHS-酯，用于标记蛋白质、肽、抗体和任何含有 -NH2 基团的分子。纯5-异构体| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2825-1mg | 1 mg | $125 | 现货 | |
| 2825-5mg | 5 mg |
$425
|
现货 | |
| 2825-25mg | 25 mg |
$1240
|
现货 | |
| 2825-50mg | 50 mg |
$1890
|
现货 | |
| 2825-100mg | 100 mg |
$2640
|
现货 |