DusQ 2 CPG 1000
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 15960 | 100 mg | – | 现货 | |
| 35960 | 500 mg |
$530
|
现货 | |
| 45960 | 1 g |
$990
|
现货 | |
| 55960 | 5 g | 请查询 | 现货 |
This modified support is intended for the synthesis of oligonucleotides with DusQ 2 quencher at the 3’ end. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides of up to 120 bases in length.
DusQ 2 is a fluorescence quencher with absorption within the range of 560 to 670 nm. It is ideal for effective FRET quenching of fluorophores with emission in this range. The quencher is also used in hybridization probes with static and combined quenching. Its quenching effectiveness does not depend on overlapping of fluorophore and quencher spectra, thus allowing for effective quenching of the broad spectrum of fluorophores, including those with emission in the red and far-red part of the spectrum. Thus, DusQ 2 can be used with such fluorophores (including but not limited to) as Cyanine3, TAMRA, ROX, Cyanine3.5, Cyanine5, Cyanine5.5.
Usage
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.
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VIC phosphoramidite, 6-isomer
VIC is an asymmetrical xanthene dye with spectral properties similar to HEX and JOE. Useful as a label for qPCR probes.
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TAMRA phosphoramidite, 5-isomer
TAMRA phosphoramidite for synthesis of 5’-labeled oligonucleotides. Pure 5-isomer.
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R6G phosphoramidite, 6-isomer
Phosphoramidite for synthesis of oligonucleotides labeled at the 5'-terminus with rhodamine 6G (R6G). Pure 6-isomer.
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General properties
| Appearance: | dark blue beads |
| Quality control: | NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| CF260: | 0.31 |
| CF280: | 0.26 |
Oligo synthesis details
| Pore size, Å: | 1000 |
| Typical loading, umol/g: | 30−50 |
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