Custom dual-labeled probe synthesis
The service of custom labeled oligonucleotide and dual-labeled probe synthesis is provided by Lumiprobe.
The probes are synthesized from dyes produced by Lumiprobe (or commercial dyes) using automated phosphoramidite chemistry. The purification is performed by PAGE and subsequent HPLC. This ensures optimal probe quality, highest purity and reproducibility.
Most fluorescent dyes are attached to the DNA using our Click-chemistry based technology. Some are introduced using Lumiprobe or other commercially available phosphoramidites.
Modification availability
| Modification | Available labeling site | ||
|---|---|---|---|
| 5'-terminus | 3'-terminus | ||
| Fluorescent dyes | |||
| FAM |  |
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| HEX | |
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| JOE | |
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| TAMRA | |
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| R6G | |
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| R110 | |
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| ROX | |
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| Cy3 | |
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| Cy3.5 | |
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| Cy5 | |
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| Cy5.5 | |
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| Cy7 | |
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| Cy7.5 | |
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| Fluorescence quenchers | |||
| Dabcyl | |
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| Other groups | |||
| Acetylene | |
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| Aminolink | |
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| Biotin | |
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*FAM, HEX, JOE, TAMRA, R6G, R110 and ROX are either pure 5- or pure 6-isomers.
*Please note that shipping cost of $50 applies to orders below $400.
Pricing
The prices do not depend on the dyes you have chosen.
| Oligo type | nmols guaranteed / price (USD) | |||||
|---|---|---|---|---|---|---|
| 3 | 20 | 50 | 100 | 200 | 500 | |
| Oligonucleotide, 30 bases or below, with HPLC/PAGE purification | N/A | $30 | $60 | $90 | $150 | $380 |
| Oligonucleotide, 31-80 bases, with HPLC/PAGE purification | N/A | $40 | $85 | $125 | $210 | $530 |
| Oligonucleotide with one modification, 30 bases or below, with HPLC/PAGE purification | $120 | $150 | $260 | Please inquire | ||
| Oligonucleotide with one modification, 31-80 bases, with HPLC/PAGE purification | $170 | $210 | $360 | |||
| Oligonucleotide with two modifications, 30 bases or below, with HPLC+PAGE purification | $220 | $280 | $440 | |||
| Oligonucleotide with two modifications, 31-80 bases, with HPLC+PAGE purification | $310 | $390 | $610 | |||
The prices are in US Dollars. World-wide flat rate shipping cost of $50 applies to orders below $400. Payment is accepted by credit card or wire transfer.
Delivery time
Delivery time depends on your order - you will be advised on inquiry. Typically, the oligos will be delivered in no more than 14 days.
How to order
Please send the sequences, oligo IDs, and requested amounts by email [email protected]
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