Cell Viability and Cytotoxicity Kit, with Calcein AM and EthD-1

显微镜观察流式细胞术细胞活力和细胞凋亡

目录号	数量	价格	提前通知时间	购买此产品
16472	100 assays	$199.00	21 天

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Cell Viability and Cytotoxicity Kit with Calcein AM and EthD-1 is a ready-to-use kit designed for the simultaneous assessment of cell viability and cytotoxicity using dual-fluorescence staining.

The kit contains two complementary probes:

Calcein AM is a nonpolar, cell-permeant fluorogenic probe. Once inside live cells, it is hydrolyzed by intracellular esterases to produce bright green fluorescent calcein, which is retained in cells with intact membranes. Fluorescence intensity correlates with metabolic activity and the number of viable cells.
Ethidium homodimer-1 (EthD-1) is a high-affinity nucleic acid—intercalating dye that is impermeant to live cells due to its relatively large molecular size and dicationic nature. In cells with compromised membrane integrity, EthD-1 passively enters the cytoplasm and nucleus, where it intercalates into double-stranded nucleic acids (DNA and RNA), resulting in a strong enhancement of fluorescence. The dye serves as an indicator of dead cells and cells in the late stages of apoptosis.

Upon co-incubation with cell cultures, viable cells exhibit green fluorescence (calcein), whereas dead and apoptotic cells fluoresce red (EthD-1). Minimal spectral overlap enables two-channel analysis without complex spectral compensation.

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