CFDA SE Cell Tracing Kit
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 16231 |
3 vials
|
– | 1 天 | |
| 26231 |
15 vials
|
$230 | 1 天 |
CFDA SE Cell Tracing Kit is used for fluorescent labeling and long-term tracing of cells.
CFDA SE ((5,6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE. This molecule is non-fluorescent until the acetate groups are cleaved by intracellular esterases resulting in the formation of a highly fluorescent fluorophore CFSE with emission in the green spectrum range (absorption max. at ~492 nm, emission max. at ~517 nm). CFSE interacts with cellular amines via its succinimidyl groups and covalently labels intracellular proteins.
CFDA SE is commonly used for in vivo and in vitro labeling of cells to analyze their proliferation as well as for cell tracking and motility assays. Inside the cell, CFDA SE shows little cytotoxicity, with minimal effects on the proliferative ability or biology of the cell. During proliferation, the label is partitioned approximately equally among the progeny so that cell division can be followed as a successive halving of the fluorescence intensity through multiple generational divisions.
CFDA SE cell tracing kit contains single-use vials of dry dye and the required amount of high-quality anhydrous DMSO. It allows the preparation of small volumes of the working staining solution, which is convenient for conducting experiments and scaling them up without unnecessary loss of the dye.
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The ready-to-use kit to label cell membranes with green fluorescent PKH2 dye for evaluating cell migration or proliferation.(5,6)-FAM diacetate NHS ester
Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.| 储存条件: | |
| 安全数据表（MSDS）: | 下载 |
| 激发/吸收极大值，纳米: | 492 |
| 发射极大值，纳米: | 517 |
| 储存时间（月: | 12 |
参考文献
- Girich, E.V.; Trinh, P.T.H; Nesterenko, L.E.; Popov, R.S.; Kim, N.Y.; Rasin, A.B.; Menchinskaya, E.S.; Kuzmich, A.S.; Chingizova, E.A.; Minin, A.S.; Ngoc, N.T.D.; Van, T.T.T.; Yurchenko, E.A.; Yurchenko, A.N.; Berdyshev, D.V. Absolute Stereochemistry and Cytotoxic Effects of Vismione E from Marine Sponge-Derived Fungus Aspergillus sp. 1901NT-1.2.2. International Journal of Molecular Sciences, 2023, 24(9), 8150. doi: 10.3390/ijms24098150
- Silchenko, A.S.; Kalinovsky, A.I.; Avilov, S.A.; Popov, R.S.; Dmitrenok, P.S.; Chingizova, E.A.; Menchinskaya, E.S.; Panina, E.G.; Stepanov, V.G.; Kalinin, V.I.; Stonik, V.A. Djakonoviosides A, A1, A2, B1–B4 — Triterpene Monosulfated Tetra- and Pentaosides from the Sea Cucumber Cucumaria djakonovi : The First Finding of a Hemiketal Fragment in the Aglycones; Activity against Human Breast Cancer Cell Lines. International Journal of Molecular Sciences, 2023, 24(13), 11128. doi: 10.3390/ijms241311128
- Zhuravleva, O.I.; Chingizova, E.A.; Oleinikova, G.K.; Starnovskaya, S.S.; Antonov, A.S.; Kirichuk, N.N.; Menshov, A.S.; Popov, R.S.; Kim, N.Y..; Berdyshev, D.V.; Chingizov, A.R.; Kuzmich, A.S.; Guzhova, I.V.; Yurchenko, A.N.; Yurchenko, E.A. Anthraquinone Derivatives and Other Aromatic Compounds from Marine Fungus Asteromyces cruciatus KMM 4696 and Their Effects against Staphylococcus aureus. Marine Drugs, 2023, 21(8), 431. doi: 10.3390/md21080431
- Silchenko, A.S.; Kalinovsky, A.I.; Avilov, S.A.; Popov, R.S.; Chingizova, E.A.; Menchinskaya, E.S.; Zelepuga, E.A.; Panina, E.G.; Stepanov, V.G.; Kalinin, V.I.; Dmitrenok, P.S. Sulfated Triterpene Glycosides from the Far Eastern Sea Cucumber Cucumaria djakonovi : Djakonoviosides C 1 , D 1 , E 1 , and F 1 ; Cytotoxicity against Human Breast Cancer Cell Lines; Quantitative Structure–Activity Relationships. Marine Drugs, 2023, 21(12), 602. doi: 10.3390/md21120602
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