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AF 488 HTag ligand

目录号 数量 价格 提前通知时间
5559-150nmol 150 nmol
$479.00
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AF 488 HTag is a highly specific fluorescent ligand designed for the covalent and irreversible labeling of HaloTag® fusion proteins in live cells, cell lysates, and fixed samples.

The HaloTag® system is based on a genetically engineered bacterial haloalkane dehalogenase that forms a covalent bond with synthetic chloroalkane ligands, making it specific and rapid. AF 488 HTag conjugate contains a chloroalkane anchor linked to a bright AF 488 fluorophore. When added to cells or samples expressing a HaloTag® fusion protein, the substrate permeates the membrane and permanently binds to the target protein. The complete absence of cross-reactivity with endogenous mammalian proteins guarantees an exceptional signal-to-noise ratio.

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General properties

Appearance: red solid
Molecular weight: 816.39
Molecular formula: C31H32ClK2N3O12S2
Solubility: DMSO, water
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
安全数据表(MSDS): 下载
产品规格

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10
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