DusQ® 1 phosphoramidite
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 1470-100mg | 100 mg | $290 | 现货 | |
| 1470-500mg | 500 mg |
$990
|
现货 | |
| 1470-1g | 1 g |
$1690
|
现货 | |
| 1470-10g | 10 g | 请查询 | 现货 |
DusQ® 1 phosphoramidite is a true dark quencher with broad absorption curve which covers the visible spectrum with maximum in green to yellow region. It is used for the synthesis of dual labeled oligonucleotide probes for qPCR bearing 5'-quencher DusQ 1 and other FRET applications for multiplexing assays. Contains a DMT protection of the hydroxymethyl group, which allows oligonucleotide purification on cartridges.
DusQ 1 has a quenching range (QR) 480 - 580 nm to construct efficiently quenched qPCR probes paired with all common reporter dyes such as FAM, TET, JOE, HEX and Cyanine3 with high quenching efficiency, and form completely non-fluorescent complexes.
Usage
Coupling: 6 minutes coupling time recommended.
Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).
Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.
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Cyanine5叠氮化物
Cyanine5 叠氮化物是一种用 Cyanine5（一种流行的荧光染料）进行点击化学标记的试剂。
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通用 CPG II 型，1000A (Universal CPG type II, 1000A)
这种通用支持物旨在用于寡核苷酸的自动合成，以加速解封闭过程中的去磷酸化过程。对于多达 120 个碱基的寡核苷酸合成，建议使用 1000 Å 的孔径。DusQ 1 CPG 500
固体支持物-可控微孔玻璃 (CPG) 500，用于合成3' 末端带有 DusQ 1 猝灭剂的寡核苷酸。与标准解封闭条件兼容。
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General properties
| Appearance: | black powder |
| Molecular weight: | 995.11 |
| CAS number: | 374591-94-3 |
| Molecular formula: | C55H63N8O8P |
| IUPAC name: | Phosphoramidous acid, bis(1-methylethyl)-, 2-[[2-[bis(4-methoxyphenyl)phenylmethoxy]ethyl][4-[[2-methoxy-5-methyl-4-[(4-methyl-2-nitrophenyl)azo]phenyl]azo]phenyl]amino]ethyl 2-cyanoethyl ester (9CI) |
| Solubility: | good in acetonitrile |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 522 |
| ε, L⋅mol−1⋅cm−1: | 27300 |
Oligo synthesis details
| Diluent: | 50% DCM in acetonitrile |
| Coupling conditions: | 6 min coupling time; 3 min oxidation time |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
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