DusQ 2 CPG 500
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 12960 | 100 mg | – | 现货 | |
| 32960 | 500 mg |
$530
|
现货 | |
| 42960 | 1 g |
$990
|
现货 | |
| 52960 | 5 g | 请查询 | 现货 |
This support is intended for automated synthesis of oligonucleotides with 3’-terminal DusQ 2 quencher. Pore size of 500 Å is recommended for the synthesis of oligonucleotides of up to 50 bases in length.
DusQ 2 is a fluorescence quencher with absorption within the range of 560 to 670 nm. It is ideal for effective FRET quenching of fluorophores with emission in this range. The quencher is also used in hybridization probes with static and combined quenching. Its quenching effectiveness does not depend very much on overlapping of fluorophore and quencher spectra, thus allowing for effective quenching of the broad spectrum of fluorophores, including those with emission in the red and far-red part of the spectrum. Thus, DusQ 2 can be used with such fluorophores (including but not limited to) as Cyanine3, TAMRA, ROX, Cyanine3.5, Quasar® 570, Pulsar® 650, Cyanine5, Quasar® 670, Cyanine5.5, and Quasar® 705.
Usage
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.
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DusQ 3 NHS ester
Amino-reactive NHS ester for the labeling with far-red quencher DusQ 3.抗坏血酸 (Ascorbic acid)
抗坏血酸是一种还原剂，可将铜 (II) 转化为用于催化点击反应的一价形式。Cyanine3叠氮化物 (Cyanine3 azide)
用于 сlick сhemistry 的 Cyanine3 荧光染料的叠氮衍生物。General properties
| Appearance: | dark blue beads |
| Quality control: | NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| CF260: | 0.31 |
| CF280: | 0.26 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−80 |
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