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Biotin-PEG4-tetrazine is a click chemistry biotinylation tool. The tetrazine group reacts extremely fast with terminal alkenes and trans-cyclooctenes via inverse electron demand Diels-Alder ligation (IEDDA). Tetrazines also react with some strained cycloalkynes.
Biotin-labeled compounds can be linked to avidin or streptavidin for further purification or detection. The biotin group is relatively small and doesn’t affect the biological activity of biotinylated proteins.
This reagent features a long PEG4-linker that separates the biotin residue from the target molecule, ensuring efficient binding to avidin or streptavidin. The linker also increases the compound’s water solubility, thereby facilitating bioconjugation.
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.