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Cyanine3 azide

目录号 数量 价格 提前通知时间
11030 100 uL, 10 mM/DMSO –   现货
31030 500 uL, 10 mM/DMSO $199 现货
41030 1 mL, 10 mM/DMSO $495 现货
A1030 1 mg –   现货
B1030 5 mg $199 现货
C1030 10 mg $297 现货
D1030 25 mg $495 现货
E1030 50 mg $895 现货
F1030 100 mg $1490 现货
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Cyanine3 dye azide for сlick сhemistry, an analog of Cy3® azide. Cy3® is one of the most broadly used fluorophores which can be detected by various fluorometers, imagers, and microscopes. Due to inherently high extinction coefficient, Cyanine3 is also easily detected by naked eye on gels, and in solution. This is non-sulfonated dye which requires organic co-solvent (DMF, DMSO, or other) for efficient labeling in water. Water-soluble version of this reagent is also available.

Product is available both as solid compound, and as 10 mM solution in DMSO which is ready to use in our recommended protocol.

Cyanine3 fluorescent properties are identical to Cy3®, and similar to DyLight 549.

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Alkyne dT phosphoramidite

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Biotin-11-UTP

Biotinylated UTP for enzymatic RNA labeling.

General properties

Appearance: red powder / solution
Molecular weight: 575.19
CAS number: 1167421-28-4 (chloride)
Molecular formula: C33H43N6OCl
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (40 mg/L = 60 uM)
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
安全数据表(MSDS): 下载
产品规格

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

产品引用

  1. Goedhart, J. Studentsourcing—Aggregating and reusing data from a practical cell biology course. PLOS Computational Biology, 2024, 20(2), e1011836. doi: 10.1371/journal.pcbi.1011836
  2. Alruwaili, M.M.; Zonneville, J.; Naranjo, M.N.; Serio, H.; Melendy, T.; Straubinger, R.M.; Gillard, B.; Foster, B.A.; Rajan, P.; Attwood, K.; Chatley, S.; Iyer, R.; Fountzilas, C.; Bakin, A.V. A synergistic two-drug therapy specifically targets a DNA repair dysregulation that occurs in p53-deficient colorectal and pancreatic cancers. Cell Reports Medicine, 2024, 5(3), 101434. doi: 10.1016/j.xcrm.2024.101434
  3. Liao, T.-W.; Huang, L.; Wilson, T. J.; Ganser, L. R.; Lilley, D. M. J.; Ha, T. Linking Folding Dynamics and Function of SAM/SAH Riboswitches at the Single Molecule Level. Nucleic Acids Research, 2023, 51(17), 8957–8969. doi: 10.1093/nar/gkad633
  4. Hernández‐Carralero, E.; Cabrera, E.; Rodríguez-Torres, G.; Hernández-Reyes, Y.; Singh, A. N.; Santa-María, C.; Fernández-Justel, J. M.; Janssens, R. C.; Marteijn, J. A.; Evert, B. O.; Mailand, N.; Gómez, M.; Ramadan, K.; Smits, V. A. J.; Freire, R. ATXN3 Controls DNA Replication and Transcription by Regulating Chromatin Structure. Nucleic Acids Research, 2023, 51(11), 5396–5413. doi: 10.1093/nar/gkad212
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