DAPI, blue fluorescent nucleic acid stain
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2996-1mL | 1 mL, 1 mg/mL in Water | $75 | 现货 | |
| 2996-50uL | 50 uL, 1 mg/mL in Water | – | 现货 | |
| 2996-1mg | 1 mg | $30 | 现货 | |
| 2996-10mg | 10 mg | $110 | 现货 |
DAPI (4′,6-diamidino-2-phenylindole) is a blue-emitting fluorescent dye that binds strongly to adenine-thymine-rich regions in DNA. It is used extensively in fluorescence microscopy, flow cytometry for chromosome staining and as a nuclear counterstain. DAPI is generally used to stain fixed cells since the dye is membrane impermeant, although the dye can enter live cells when used at high concentrations.
When bound to double-stranded DNA, DAPI exhibits ~20-fold enhancement of fluorescence with an absorption maximum at 358 nm and emission maximum at 461 nm. DAPI also binds to RNA, though it is not as strongly fluorescent. When bound to RNA, its emission spectrum shifts to around 500 nm.
There is a slight fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and GFP. Use spectral unmixing if extremely precise image analysis is required.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting DNA and detecting annealed probes in blotting applications by specifically visualizing the double-stranded complex. Also, DAPI staining is a sensitive and specific detection method for mycoplasma.
顾客还购买了这个产品
dsGold 核酸凝胶染色液，10000× (dsGold Nucleic Acid Gel Staining Solution, 10000×)
一种高灵敏度染色剂，用于电泳凝胶中的 dsDNA、ssDNA 和 RNA 染色。磺基-Cyanine5 NHS 酯 (sulfo-Cyanine5 NHS ester)
水溶性磺基-Cyanine5 琥珀酰亚胺酯，用于在水相中标记各种含胺分子。sulfo-Cyanine5 tyramide
A tyramide derivative of sulfo-Cyanine5, a far-red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.General properties
| Appearance: | yellow solid / solution |
| Molecular weight: | 350.25 |
| CAS number: | 28718-90-3 |
| Molecular formula: | C16H17Cl2N5 |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 355 |
| ε, L⋅mol−1⋅cm−1: | 28800 |
| Emission maximum, nm: | 460 |
产品引用
- Potapenko, E.Y..; Kashko, N.D.; Knorre, D.A. Spontaneous Mutations in Saccharomyces cerevisiae mtDNA Increase Cell-to-Cell Variation in mtDNA Amount. International Journal of Molecular Sciences, 2023, 24(24), 17413. doi: 10.3390/ijms242417413
您的购物车是空的
$ 
