DDTT Sulfurizing Reagent
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 3179-1g | 1 g | – | 现货 | |
| 3179-5g | 5 g | $245 | 现货 | |
| 3179-25g | 25 g |
$1150
|
现货 | |
| 3179-50g | 50 g |
$1800
|
现货 | |
| 3179-100g | 100 g | 请查询 | 现货 |
DDTT is a sulfurizing reagent used to prepare phosphorothioate linkages, which increase the stability and nuclease resistance of nucleic acids, especially of RNA. It is suitable for automated synthesizers employing standard phosphoramidites for a large-scale synthesis of oligonucleotides.
DDTT demonstrates faster kinetics and higher stability in solution, compared to other sulfurizing reagents such as tetraethylthiuram disulfide (TETD) and Beaucage reagent, respectively. DDTT is commonly applied as a 0.05 M or 0.1 M solution in pyridine/acetonitrile and has a lifetime of up to 6 months.
Usage
Coupling: Sulfurization step must come before capping step. The optimum sulfurizing time for DDTT Sulfurizing Reagent, 0.05 M solution in pyridine/acetonitrile: 1 min for DNA or 6 min for RNA.
Deprotection: standard conditions.
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Cyanine5.5 NHS酯 (Cyanine5.5 NHS ester)
Cyanine5.5 NHS 是一种远红/近红外胺反应性染料。
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JOE NHS ester, 5-isomer
JOE is a fluorescent dye with emission in the yellow part of the spectrum. This derivative is an activated NHS ester for conjugation with amines.通用 CPG II 型，1000A (Universal CPG type II, 1000A)
这种通用支持物旨在用于寡核苷酸的自动合成，以加速解封闭过程中的去磷酸化过程。对于多达 120 个碱基的寡核苷酸合成，建议使用 1000 Å 的孔径。General properties
| Appearance: | lemon yellow crystals |
| Molecular weight: | 205.33 |
| CAS number: | 1192027-04-5 |
| Molecular formula: | C5H7N3S3 |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Deprotection conditions: | identical to protected nucleobases |
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