DAF-FM (4-Amino-5-Methylamino-2',7'-Difluorofluorescein)

Microscopy Flow cytometry ROS sensing

目录号	数量	价格	提前通知时间	购买此产品
SB100	50 ug	$22	12 天 (起定量:10 个起)
1B100	1 mg	$365	12 天

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DAF-FM (4-Amino-5-Methylamino-2',7'-Difluorofluorescein) is a cell-impermeant, fluorescent probe for detecting and quantifying low concentrations of nitric oxide (NO). DAF-FM does not need to be activated by cytosolic enzymes and is suitable to detect NO in extracellular matrix.

The fluorescence quantum yield of DAF-FM is ~0.005, but it increases about 160-fold to ~0.81 after reacting with NO and forming a fluorescent benzotriazole (excitation/emission maxima at 495/515 nm) .

The NO detection limit of DAF-FM (~3 nM) is more sensitive than that of DAF-2 (~5 nM). The fluorescence of the NO adduct of DAF-FM is independent of pH above pH 5.5. Moreover, the NO adduct of DAF-FM demonstrates a significantly enhanced photostability compared to that of DAF-2, ensuring reliable results and additional time for imaging.

DAF-FM should be dissolved in DMSO and then used to prepare a working solution. Buffers containing bovine serum albumin (BSA) or phenol red can affect the fluorescence and should be used cautiously.

The cell-permeant version of DAF FM — DAF-FM DA is also available.

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DAF-FM 二乙酸酯（4-氨基-5-甲氨基-2',7'-二氟荧光素二乙酸酯）[DAF-FM diacetate (4-amino-5-methylamino-2',7'-difluorofluorescein diacetate)]

用于检测和定量低浓度一氧化氮 (NO)的细胞渗透性荧光探针。

6-Carboxy-H2DCFDA (6-carboxy-2',7'-dichlorodihydrofluorescein)

A reagent for detecting reactive oxygen species (ROS) in living cells. This carboxylated H2DCFDA analog has two additional negative charges that impede its leakage out of the cell.

General properties

Appearance:	yellow solid
Molecular weight:	412.35
CAS number:	254109-20-1
Molecular formula:	C₂₁H₁₄F₂N₂O₅
Solubility:	good in methanol, DMSO, DMF and water; limited in water; poor in acetonitrile and methylene chloride
Quality control:	NMR ¹H and HPLC-MS (90+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
安全数据表（MSDS）:	下载
	产品规格

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.

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