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JOE phosphoramidite, 5-isomer

目录号 数量 价格 提前通知时间
C1160 100 mg –   现货
D1160 250 mg $165 现货
F1160 1 g $575 现货
G1160 5 g $2230 现货
H1160 10 g $3300 现货
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JOE is a xanthene dye, fluorescein derivative possessing two chloro- and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA.

The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains a pure isomer of 5-JOE dye.

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DusQ 1 phosphoramidite

A black quencher dye phosphoramidite for the synthesis of oligonucleotide probes labeled with DusQ 1 quenchers at 5’-terminal. Useful for HEX, JOE, ROX, Cyanine3.
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General properties

Appearance: colorless solid
Molecular weight: 972.88
Molecular formula: C48H60N3Cl2O12P
Solubility: good in DCM, acetonitrile
Quality control: NMR 1H, 31P, HPLC-MS (95%), functional testing
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
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产品规格

Spectral properties

Excitation/absorption maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 554
Fluorescence quantum yield: 0.61
CF260: 0.36
CF280: 0.28

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
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