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Copper(II)-THPTA catalytic buffer, 1.5x

Click chemistry

目录号	数量	价格	提前通知时间
B5150	1 mL	$30	现货
K5150	10 mL	$120	现货
S5150	50 mL	$490	现货

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Catalytic buffer is suitable for coupling of azide- or alkyne-modified protein with alkyne- or azide- bearing dye via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). THPTA ligand accelerates the reaction rate due to the stabilization of a catalytically active Cu (I). Moreover, the presence of water-soluble THPTA allows the protein labeling to be run in aqueous solution and, by chelating free copper, minimizes the generation of ROS (reactive oxygen species) and undesired damage of proteins. Aminoguanidine prevents connections of reactive aldehydes, which are products of dehydroascorbate hydrolysis, with arginine, N-terminal cysteine, and lysine side-chains. Ready-to-use 1.5× buffer provides all the necessary reagents to perform the CuAAC reaction, except a reagent for the reduction of Cu (II) into catalytically active Cu (I). As a reducing agent we recommend ascorbic acid.

Buffer composition: Cu (II), triethylammonium acetate buffer pH 6.8, THPTA ligand, aminoguanidine

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General properties

Storage conditions:	Storage: 12 months after receival at +4 or -20°C in the dark. Transportation: at room temperature for up to 3 weeks.
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	产品规格

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.