Copper(II)-THPTA catalytic buffer, 1.5x
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| B5150 | 1 mL | $30 | 现货 | |
| K5150 | 10 mL | $120 | 现货 | |
| S5150 | 50 mL |
$490
|
现货 |
Catalytic buffer is suitable for coupling of azide- or alkyne-modified protein with alkyne- or azide- bearing dye via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). THPTA ligand accelerates the reaction rate due to the stabilization of a catalytically active Cu (I). Moreover, the presence of water-soluble THPTA allows the protein labeling to be run in aqueous solution and, by chelating free copper, minimizes the generation of ROS (reactive oxygen species) and undesired damage of proteins. Aminoguanidine prevents connections of reactive aldehydes, which are products of dehydroascorbate hydrolysis, with arginine, N-terminal cysteine, and lysine side-chains. Ready-to-use 1.5x buffer provides all the necessary reagents to perform the CuAAC reaction, except a reagent for the reduction of Cu (II) into catalytically active Cu (I). As a reducing agent we recommend ascorbic acid.
Buffer composition: Cu (II), triethylammonium acetate buffer pH 6.8, THPTA ligand, aminoguanidine
顾客还购买了这个产品
Azide-PEG3-azide
Triethylene glycol diazide is a bifunctional crosslinker bearing two azide groups. Suitable for click chemistry.磺基- Cyanine3炔基 (sulfo-Cyanine3 alkyne)
一种用于铜催化点击化学的水溶性染料炔烃。荧光团 sulfo-Cyanine3 是一种用于 Cy3® 通道的明亮且光稳定的染料。General properties
| Storage conditions: | Storage: 12 months after receival at +4 or -20°C in the dark. Transportation: at room temperature for up to 3 weeks. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
您的购物车是空的
$ 
