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AF 594 streptavidin

目录号 数量 价格 提前通知时间
A68S0 100 ug –   现货
168S0 1 mg $185 现货
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Streptavidin is a tetrameric biotin-binding protein derived from the bacterium Streptomyces avidinii. Streptavidin binds up to four biotin molecules with high affinity and selectivity via multiple hydrogen bonds and van der Waals interactions. Due to the lack of carbohydrate modifications and a near-neutral pI, streptavidin exhibits less nonspecific binding than another biotin-binding protein — avidin. Streptavidin also has high thermostability and resistance against extreme pH, denaturing agents, and enzymatic degradation, allowing using this protein under various experimental conditions.

Fluorescent conjugates of streptavidin are commonly used as a second-step reagent for specific detection of a variety of biotin-labeled biomolecules, such as proteins (antibodies, etc.), nucleic acids, lipids, and other molecules in indirect immunofluorescent staining, western blots, flow cytometry, microplate assays, and other detection techniques.

This streptavidin is a lyophilized conjugate with AF 594, a bright, photostable red fluorophore with spectral characteristics similar to Texas Red (absorption max. at 586 nm, emission max. at 613 nm).

The recommended concentration range for use is 0.5-10 µg/mL. Avoid using biotin-containing solutions (some serums, RPMI 1640, etc.) as diluents.

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AF 594 染料的红色荧光 NHS-酯,用于标记蛋白质、肽、抗体和任何含有 -NH2 基团的分子。纯5-异构体
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Biocytin hydrochloride

Lysine derivative of biotin for conjugation with avidin or streptavidin, neuroanatomical tracer.

sulfo-Cyanine5 streptavidin

sulfo-Cyanine5 conjugate of streptavidin, a high-affinity biotin-binding protein, for immunofluorescence, western blots, microplate assays, and other applications use.

General properties

Appearance: purple solid
Solubility: good in water
Storage conditions: Transportation: at room temperature for 1 week. Store at -20°С 9 months.
安全数据表(MSDS): 下载
产品规格

Spectral properties

Excitation/absorption maximum, nm: 586
ε, L⋅mol−1⋅cm−1: 105000
Emission maximum, nm: 613
Fluorescence quantum yield: 0.77
CF260: 0.28
CF280: 0.51
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