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sulfo-Cyanine3.5 carboxylic acid

目录号 数量 价格 提前通知时间
12390 1 mg $110 现货
22390 5 mg $210 现货
42390 25 mg $410 现货
52390 50 mg $695 现货
62390 100 mg $1190 现货
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sulfo-Cyanine3.5 is a cyanine fluorophore with fluorescence in the orange spectrum range. Its absorption and emission spectra are between those of Cyanine3 and Cyanine5.

This dye is a sulfonated form of Cyanine3.5 (contains four sulfo groups), so it has good solubility and can be used in reactions without adding organic solvents.

This reagent is a derivative with a free carboxyl group. Because of its inactivity in physiological conditions, sulfo-Cyanine3.5 carboxylic acid derivative can be used as a negative control in experiments with active dye derivatives. The carboxylic acid can be activated with carbodiimides (e. g. EDAC) and react with hydrazines, hydroxylamines, and amines.

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AF 647 carboxylic acid

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sulfo-Cyanine3.5 NHS ester

Amine-reactive activated ester of water-soluble, sulfonated sulfo-Cyanine3.5 dye.

General properties

Appearance: dark colored solid
Molecular weight: 991.26
Molecular formula: C38H37N2K3O14S4
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
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Spectral properties

Excitation/absorption maximum, nm: 576
ε, L⋅mol−1⋅cm−1: 139000
Emission maximum, nm: 603
Fluorescence quantum yield: 0.11
CF260: 0.16
CF280: 0.17
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