ssGreen RNA Gel Staining Solution, 10,000×
目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
---|---|---|---|---|
A2510 | 50 uL | – | 现货 | |
12510 | 500 uL | $385 | 现货 |
ssGreen® is one of the most sensitive stains for post-electrophoresis staining of RNA and single-stranded DNA (ssDNA) in agarose or polyacrylamide gels. The fluorescence quantum yield of the ssGreen/RNA complex is more than 7× higher than that of the ethidium bromide/RNA complex. Although ssGreen is not selective for RNA staining, the dye exhibits about 1.5× greater quantum yield when bound to RNA than double-stranded DNA, which makes it unique among all nucleic acid dyes.
ssGreen staining is compatible with denaturing gels. On agarose/formaldehyde and polyacrylamide/urea gels, the sensitivity of ssGreen is slightly reduced but still superior to that of ethidium bromide. Staining agarose/formaldehyde gels with ssGreen does not interfere with transfer of RNA to filters or subsequent hybridization in Northern blot analysis as long as 0.1%–0.3% SDS is included in prehybridization and hybridization buffers.
Staining gels with ssGreen has fewer steps than those with ethidium bromide. Because the fluorescence of ssGreen/RNA complexes is not quenched by formaldehyde or urea, there is no need to wash these denaturants out of gels before staining. Also, ssGreen stain has a low intrinsic fluorescence, allowing gel viewing and photographing without preliminary removing unbound dye.
计算器
顾客还购买了这个产品
DiI,亲脂性示踪剂 (DiI, lipophilic tracer)
用于膜标记和细胞示踪的橙红色荧光亲脂染料。dsSafe Nucleic Acid Gel Staining Solution, 10,000×
Fluorescent dye for DNA and RNA gel visualization, the best and safer alternative to ethidium bromide.Cyanine3酰肼 (Cyanine3 hydrazide)
Cyanine3酰肼是可用于 Cy3 通道的羰基反应活性染料。General properties
Appearance: | dark orange solution |
Molecular weight: | 581.52 |
CAS number: | 172827-25-7 |
Molecular formula: | C28H28IN3OS |
Quality control: | UV-Vis abs |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
安全数据表(MSDS): | 下载 |
产品规格 |
Spectral properties
Excitation/absorption maximum, nm: | 483 (complex) |
Emission maximum, nm: | 518 (complex) |