AF 594 tyramide
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 3804-1mg | 1 mg | $150 | 现货 |
Thyramide signal amplification (TSA) is the most versatile and effective way to enhance the intensity of the fluorescent signal, used in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). The TSA method is based on the ability of horseradish peroxidase (HRP) in the presence of low concentrations of hydrogen peroxide to convert a labeled tyramine-containing substrate into an oxidized, highly reactive free radical that covalently binds to the tyrosine residues of protein molecules located next to it.
Compared to conventional procedures, the TSA method increases the sensitivity of immunofluorescent detection of target molecules by more than 100 times, making it particularly suitable for detecting low-concentration targets. In applications where increased detection sensitivity is not required, TSA can significantly reduce antibody or probe concentrations without loss of signal intensity, thereby reducing background staining due to cross-reactivity or non-specific binding of antibodies.
Since the binding of the tyramide label is covalent, tyramides of different dyes can be used in several sequential rounds of TSA staining to detect multiple targets in the same sample.
This tyramide is a conjugate of the water-soluble red fluorescent dye AF 594. AF 594 tyramide is a component of many tyramide signal amplification (TSA) kits. It can be used with any antibody or other molecules (streptavidin, etc.) conjugated to HRP to stain cells and tissues by immunofluorescence methods.
计算器
顾客还购买了这个产品
Copper(II)-TBTA complex
A catalyst for click reaction. A stable copper(II) complex which must be converted to monovalent copper(I) in situ using reducing agents. The complex can be used to prepare a reaction buffer of any composition.LumiMount Fluorescence Mounting Medium
An aqueous anti-fade mounting medium for coverslipping cells and tissues stained with various fluorescent dyes.AF 488 tyramide
A tyramide derivative of AF 488, a green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.General properties
| Appearance: | dark-blue crystals |
| Molecular weight: | 943.15 |
| Molecular formula: | C49H58N4O11S2 |
| Solubility: | soluble in water, DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 586 |
| ε, L⋅mol−1⋅cm−1: | 105000 |
| Emission maximum, nm: | 613 |
| Fluorescence quantum yield: | 0.77 |
| CF260: | 0.28 |
| CF280: | 0.51 |
您的购物车是空的
$ 
