TAMRA CPG 500, 5-isomer
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2792-100mg | 100 mg | $95 | 现货 | |
| 2792-500mg | 500 mg | $295 | 现货 | |
| 2792-1g | 1 g |
$469
|
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| 2792-5g | 5 g | 请查询 | 现货 |
Controlled pore glass solid support with TAMRA for the synthesis of 3'-labeled oligonucleotides.
TAMRA CPG supports allow for the introduction of a reporter/quencher to be used in probe-based assays, like TaqMan probes for real-time PCR quantification and FRET experiments, which require that oligonucleotides be doubly labeled.
The proposed solid support 500 Å provides an optimal yield of oligonucleotides up to 50 mer. The TAMRA dye is not stable in the presence of ammonium, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.
Usage
Coupling: standard time depending on the first monomer.
Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 h at 60 °C, then cool down to room temperature.
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TAMRA phosphoramidite, 5-isomer
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Cyanine 5.5叠氮化物 (Cyanine5.5 azide)
用于点击化学的远红/近红外荧光 Cyanine5.5 染料衍生物。Cyanine5.5亚磷酰胺 (Cyanine5.5 phosphoramidite)
Amidite 用于合成5' 末端带有Cyanine5.5染料的寡核苷酸和 qPCR 探针。适用于多重 qPCR。
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General properties
| Appearance: | deep pink beads |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.32 |
| CF280: | 0.19 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−70 |
产品引用
- Bauer, I.; Ilina, E.; Zharkov, T.; Grigorieva, E.; Chinak, O.; Kupryushkin, M.; Golyshev, V.; Mitin, D.; Chubarov, A.; Khodyreva, S.; Dmitrienko, E. Self-Penetrating Oligonucleotide Derivatives: Features of Self-Assembly and Interactions with Serum and Intracellular Proteins. Pharmaceutics, 2023, 15(12), 2779. doi: 10.3390/pharmaceutics15122779
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