TAMRA CPG 500, 5-isomer
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2792-100mg | 100 mg | $95 | 现货 | |
| 2792-500mg | 500 mg | $295 | 现货 | |
| 2792-1g | 1 g |
$469
|
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| 2792-5g | 5 g | 请查询 | 现货 |
Controlled pore glass solid support with TAMRA for the synthesis of 3'-labeled oligonucleotides.
TAMRA CPG supports allow for the introduction of a reporter/quencher to be used in probe-based assays, like TaqMan probes for real-time PCR quantification and FRET experiments, which require that oligonucleotides be doubly labeled.
The proposed solid support 500 Å provides an optimal yield of oligonucleotides up to 50 mer. The TAMRA dye is not stable in the presence of ammonium, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.
Usage
Coupling: standard time depending on the first monomer.
Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 h at 60 °C, then cool down to room temperature.
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Biotin-PEG4-azide
Biotin azide is a reagent for click chemistry labeling with biotin, a well-known affinity probe to streptavidin.铜(II)-TBTA络合物 (Copper(II)-TBTA complex)
点击反应的催化剂。一种稳定的铜 (II) 络合物，使用还原剂，可原位转化为一价铜 (I)。该络合物可用于制备任何组成的反应缓冲液。TAMRA phosphoramidite, 5-isomer
TAMRA phosphoramidite for synthesis of 5’-labeled oligonucleotides. Pure 5-isomer.
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General properties
| Appearance: | deep pink beads |
| Quality control: | NMR 1H and loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.32 |
| CF280: | 0.19 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−70 |
产品引用
- Bauer, I.; Ilina, E.; Zharkov, T.; Grigorieva, E.; Chinak, O.; Kupryushkin, M.; Golyshev, V.; Mitin, D.; Chubarov, A.; Khodyreva, S.; Dmitrienko, E. Self-Penetrating Oligonucleotide Derivatives: Features of Self-Assembly and Interactions with Serum and Intracellular Proteins. Pharmaceutics, 2023, 15(12), 2779. doi: 10.3390/pharmaceutics15122779
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