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TFA-amino modifier CPG 500

目录号 数量 价格 提前通知时间
11860 100 mg $60 现货
21860 250 mg $120 现货
31860 500 mg $230 现货
41860 1 g $380 现货
51860 5 g $1450 5 天
61860 10 g $2465 现货
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This high load, 500 Å controlled pore glass solid support is designed for the synthesis of oligonucleotides containing up to 50 bases with a 3'-amino group. Amino group is protected with trifluoroacetyl (TFA) protection which is easily removed under standard deblock conditions.

The reagent is based on hydroxyprolinol core - a universal non-nucleoside structure that is naturally 100% chiral (no isomers formed upon condensation), and stable to all conditions of oligonucleotide synthesis and deblock.

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R6G azide, 5-isomer

Rhodamine 6G is a xanthene dye that has been used for DNA sequencing and qPCR. This azide derivative is used for the attachment of the dye to DNA and other molecules by means of click chemistry.

R6G phosphoramidite, 6-isomer

Phosphoramidite for synthesis of oligonucleotides labeled at the 5'-terminus with rhodamine 6G (R6G). Pure 6-isomer.
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Inosine (dI) phosphoramidite

Dimethoxytrityl deoxyinosine (Dmt-dI) phosphoramidite for the synthesis of modified DNA oligonucleotides.

General properties

Appearance: off-white beads
Quality control: NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement
Storage conditions: Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.
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产品规格

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50−80
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases

产品引用

  1. Taskova, M.; Uhd, J.; Miotke, L.; Kubit, M.; Bell, J.; Ji, H.P.; Astakhova, K. Tandem Oligonucleotide Probe Annealing and Elongation To Discriminate Viral Sequence. Analytical Chemistry, 2017, 89(8), 4363–4366. doi: 10.1021/acs.analchem.7b00646
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