TOR-G4, G-quadruplexes fluorescent probe
目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
---|---|---|---|---|
A5510 | 50 uL, 5 mM/DMSO | $149 | 12 天 | |
15510 | 500 uL, 5 mM/DMSO | $390 | 12 天 | |
4357-1g | 1 g | 请查询 | 21 天 |
G-quadruplexes (G4s) are secondary structures that form in DNA and RNA through noncanonical hydrogen bonding between four guanine bases [1,2]. In the nucleus, DNA G4s have been associated with epigenetic regulation of gene expression through their interactions with regulatory proteins, such as transcription factors and chromatin modifiers [3,4]. RNA G4s have been linked to RNA splicing, transport, and translation regulation, as well as RNA-mediated stress responses in the cytoplasm [5-7].
TOR-G4 is a thiazole orange derivative, a newly synthesized G4 fluorescent probe [8]. It is a small-molecule alternative to immunochemistry with G4-specific antibodies. TOR-G4 allows the visualization of G4s based on changes to the fluorescence lifetime of a probe upon nucleic acid binding. The lifetime of TOR-G4 is highest in the presence of G4s and lower for other sequences. Within cells, TOR-G4 is primarily colocalized with RNA in the cytoplasm and nucleoli, making it the first lifetime-based probe validated for exploring the emerging roles of RNA G4s in cell functioning. TOR-G4 is suitable for imaging RNA G4s via FLIM [8].
[1] Trends in Chemistry 2, 123 (2020); [2] Nat Rev Mol Cell Biol 21, 459 (2020); [3] Nucleic Acids Res 49, 8419 (2021); [4] Trends Genet 35, 29 (2019); [5] Nucleic Acids Res 48, 12534 (2020); [6] Trends Biochem Sci 46, 270 (2021); [7] Nucleic Acids Res 49, 5426 (2021); [8] J Am Chem Soc 146, 1009 (2024).
General properties
Appearance: | black solid |
Molecular weight: | 607.56 |
Molecular formula: | C30H30IN3OS |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
安全数据表(MSDS): | 下载 |
产品规格 |
Spectral properties
Excitation/absorption maximum, nm: | 540 |
Emission maximum, nm: | 660 |