Exo-BCN-PNP
目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
---|---|---|---|---|
4043-100mg | 100 mg | $280 | 现货 | |
4043-500mg | 500 mg | $990 | 现货 |
BCN-PNP carbonate is a tool to introduce BCN moety via carbamate linkage with primary amino groups of substrate. Reaction conditions is similar to NHS-amino linkage but PNP ester provides less off-target hydrolysis and higher conjugation yield forming hydrolytically stable bond with substrate.
Bicyclononyne (BCN) is one of the most reactive cyclooctynes for copper-free click chemistry. Both exo- and endo- isomers are active and have near the same power of reactions konstants [1,2]. BCN reacts even faster than DBCO [1] with aromatic azides and comparing to dibenzofused systems has two advantages. First, it contains a plane of symmetry which prevents the formation of mixtures of stereoisomeric products. Second, BCN provides lower lipophilicity which is typically more beneficial when reaction is performed in aqueous solutions. Unlike DBCO, BCN reactivity is not only limited to azides (SPAAC) and nitrones (SPANC), but also covers tetrazines (IEDDA) [2] and more recently tetrazoles (photoclick) [3] providing exceptional reaction rates.
[1] Jan Dommerholt et al. Readily Accessible Bicyclononynes for Bioorthogonal Labeling and Three-Dimensional Imaging of Living Cells. Angewandte Chemie. 2010. 49(49). 9422-9425.
[2] Wagner et al. Origin of Orthogonality of Strain-Promoted Click Reactions. Chemistry. 2015. 21(35).12431-12435.
[3] Gangam Srikanth Kumar et al. Superfast Tetrazole–BCN Cycloaddition Reaction for Bioorthogonal Protein Labeling on Live Cells. Journal of the American Chemical Society. 2022. 144(1). 57-62.
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DBCO-STP酯 (DBCO-STP ester)
一种活化酯用于蛋白质/抗体/肽的修饰,引入到无铜点击化学反应。DBCO-NHS 酯
偶氮二苯并环辛炔 (DBCO、ADIBO) 试剂用于应变促进的无铜点击化学 (SPAAC)。该试剂含有 NHS 酯官能团,用于将环辛炔连接到各种分子上。DBCO-PEG6-NHS ester
A heterobifunctional linker with DBCO and NHS ester function groups flanking PEG6 (hexaethylene glycol).General properties
Appearance: | off-white solid |
Molecular weight: | 315.32 |
Molecular formula: | C17H17NO5 |
Solubility: | good in DMF, DMSO, acetonitrile |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
安全数据表(MSDS): | 下载 |
产品规格 |