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R6G NHS ester, 5-isomer

目录号 数量 价格 提前通知时间
14220 1 mg
$110
现货
24220 5 mg
$210
现货
44220 25 mg
$410
现货
54220 50 mg
$695
现货
64220 100 mg
$1190
现货
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Rhodamine 6G (R6G) is a xanthene dye of rhodamine series that has been used for the labeling of oligonucleotides and DNA for quite a long time. Just like other xanthenes, R6G comes as two isomers, 5- and 6-isomer. Even though their absorption and emission spectra are virtually indistinguishable, the isomers need to be separated to avoid separation of the labeled molecules during their purification, i.e. double HPLC peaks and double spots on electrophoresis.

This is an amine reactive NHS ester derived from a pure 5-isomer of R6G. Rhodamine 6G is very bright (even used as a quantum yield standard, yield 0.95). The dye is soluble in organic solvents, such as DMF, or DMSO. They can be used as co-solvents for the labeling reactions.

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ROX NHS ester, 5-isomer

Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.
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EdU(5-乙炔基-2'-脱氧尿苷)

EdU 是一种核苷,通过细胞内的酶掺入到复制的 DNA 中。之后,可以通过与荧光染料叠氮化物的点击化学反应来显色细胞中含有的DNA,以揭示细胞增殖情况。

FAM NHS 酯,6-异构体 (FAM NHS ester, 6-isomer)

活化的荧光素 N-羟基琥珀酰亚胺酯 (FAM),纯 6-异构体。

General properties

Appearance: dark colored solid
Molecular weight: 555.58
Molecular formula: C31H29N3O7
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (80%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
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产品规格

Spectral properties

Excitation/absorption maximum, nm: 519
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 546
Fluorescence quantum yield: 0.95
CF260: 0.18
CF280: 0.17
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