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R6G NHS ester, 5-isomer

目录号 数量 价格 提前通知时间
14220 1 mg $110 现货
24220 5 mg $210 现货
44220 25 mg $410 现货
54220 50 mg $695 现货
64220 100 mg $1190 现货
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Rhodamine 6G (R6G) is a xanthene dye of rhodamine series that has been used for the labeling of oligonucleotides and DNA for quite a long time. Just like other xanthenes, R6G comes as two isomers, 5- and 6-isomer. Even though their absorption and emission spectra are virtually indistinguishable, the isomers need to be separated to avoid separation of the labeled molecules during their purification, i.e. double HPLC peaks and double spots on electrophoresis.

This is an amine reactive NHS ester derived from a pure 5-isomer of R6G. Rhodamine 6G is very bright (even used as a quantum yield standard, yield 0.95). The dye is soluble in organic solvents, such as DMF, or DMSO. They can be used as co-solvents for the labeling reactions.

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General properties

Appearance: dark colored solid
Molecular weight: 555.58
Molecular formula: C31H29N3O7
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
安全数据表(MSDS): 下载
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Spectral properties

Excitation/absorption maximum, nm: 519
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 546
Fluorescence quantum yield: 0.95
CF260: 0.18
CF280: 0.17
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