Annexin V-AF 488 Apoptosis Detection Kit, with Propidium Iodide

目录号	数量	价格	提前通知时间	购买此产品
11172	10 assays	–	1 天
21172	50 assays	$215 $169	1 天

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Annexin V (or Annexin A5) is a member of the phospholipid-binding annexin family of intracellular proteins. In flow cytometry and fluorescent microscopy, Annexin V is commonly used to detect apoptotic cells by its ability to specifically bind to phosphatidylserine (PS) in a calcium-dependent manner. The method was first reported by Koopman et al. (1994) [1].

In healthy cells, PS is normally retained in the inner leaflet of the plasma membrane. The early stage of apoptosis is accompanied by a loss of membrane phospholipid asymmetry, resulting in the exposure of PS at the cell's surface. In conjunction with vital dyes such as propidium iodide (PI), fluorochrome-labeled Annexin V can distinguish between healthy (Annexin⁻PI⁻), apoptotic (Annexin⁺PI⁻), and necrotic (Annexin⁺PI⁺) cells [2].

The ready-to-use kit contains all necessary reagents to label apoptotic cells with Annexin V-AF 488 conjugate and PI.

AF 488 is bright, photostable, and hydrophilic sulfonated rhodamine fluorophore. The dye emits in the green channel. The spectrum is similar with FITC. Its absorption max. is at 495 nm, the emission max. is at 519 nm.

PI is a membrane-impermeable DNA stain, making it useful for differentiating necrotic, apoptotic, and healthy cells based on membrane integrity. After DNA binding, the dye emits in the orange-red channel. Its absorption max. is at 535 nm, the emission max. is at 617 nm.

[1] Koopman G. et al. Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood. 1994. 84(5). P.1415-1420.

[2] Martin S.J. et al. Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J Exp Med. 1995. 182(5). P.1545-1556.

染色示例

Annexin V-AF488 细胞凋亡检测试剂盒（21172，Lumiprobe）用于荧光显微镜观察 (20x) [Fluorescence microscopy (20x) using Annexin V-AF488 Apoptosis Detection Kit (21172, Lumiprobe)]