Annexin V-AF 488 conjugate
目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
---|---|---|---|---|
11515 | 1 ug | – | 现货 | |
21515 | 5 ug | $119 | 现货 |
Annexin V (or Annexin A5) belongs to the phospholipid-binding Annexin family of intracellular proteins. In flow cytometry and fluorescent microscopy, Annexin V is commonly used to detect apoptotic cells by its ability to specifically bind to phosphatidylserine, which migrates from the inner to the outer leaflet of the plasma membrane during the early stages of apoptosis.
This Annexin V is a lyophilized conjugate with AF 488, a bright, photostable green fluorophore with spectral characteristics similar to FITC (absorption max. at 495 nm, emission max. at 519 nm).
Annexin V-AF 488 staining alone does not segregate the populations of apoptotic and necrotic cells. For this, additional staining with nuclear dyes that do not penetrate living cells — with propidium iodide or YODi-3 is required. For this purpose, you can also use our ready-to-use Annexin V-AF 488 apoptosis detection kit.
顾客还购买了这个产品
磺基-Cyanine5 NHS 酯 (sulfo-Cyanine5 NHS ester)
水溶性磺基-Cyanine5 琥珀酰亚胺酯,用于在水相中标记各种含胺分子。EDC(N-乙基-N'-(3-二甲基氨基丙基)碳二亚胺),偶联等级
水溶性碳二亚胺,用于羧酸与胺的偶联。Ascorbic acid
Ascorbic acid is a reducing agent converting copper(II) into a monovalent form that catalyzes the click reaction.General properties
Appearance: | dark orange solid |
Solubility: | good in water |
Storage conditions: | Transportation: at room temperature for 1 week. Store at -20°С 9 months. |
安全数据表(MSDS): | 下载 |
产品规格 |
Spectral properties
Excitation/absorption maximum, nm: | 495 |
ε, L⋅mol−1⋅cm−1: | 71800 |
Emission maximum, nm: | 519 |
Fluorescence quantum yield: | 0.91 |
CF260: | 0.16 |
CF280: | 0.10 |
产品引用
- Fayzullina, D.; Yakushov, S.; Kantserova, K.; Belyaeva, E.; Aniskin, D.; Tsibulnikov, S.; Fayzullina, N.; Kalinin, S.; Romantsova, O.; Timashev, P.S.; Schroeder, B.A.; Ulasov, I.V. Carbonic Anhydrase Inhibitors Induce Ferroptosis through Inhibition of AKT/FTH1 Signaling in Ewing Sarcoma Tumor Cells. Cancers, 2023, 15(21), 5225. doi: 10.3390/cancers15215225