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Protein Biotinylation Kit

目录号 数量 价格 提前通知时间
BB21-1rxn
/ for 100 ug IgG
1 rxn
$156.00
1 天
BB21-10rxn
/ for 10× 100 ug IgG
10 rxn
$389.00
21 天
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Protein Biotinylation Kit is a ready-to-use kit for covalent labeling of purified proteins, peptides, and antibodies with biotin using activated NHS-ester biotin. The kit provides fast, reproducible, and gentle biotinylation of primary amino groups (the ε-amino groups of lysine residues and N-terminal amino groups) without significantly affecting the protein’s biological activity.

The optimized protocol enables biotinylation in a short time and allows control over the degree of labeling by adjusting the reagent/protein ratio. The resulting biotinylated conjugates are fully compatible with streptavidin-biotin systems and with standard detection and affinity-immobilization methods.

Key Features

  • One-step biotinylation of primary amino groups using NHS-biotin
  • Fast reaction at physiological and slightly alkaline pH
  • Suitable for most purified peptides, proteins, and antibodies
  • Does not require prior protein modification
  • Preserves native protein properties due to mild reaction conditions
  • Compatible with ELISA, Western blotting, enzyme immunoassay, immunochemistry, flow cytometry, and affinity methods

Applications

  • Preparation of biotinylated antibodies and antigens
  • Streptavidin/avidin-based immunoassays
  • Affinity immobilization of proteins
  • Cellular and biochemical research
  • Development of diagnostic reagents

We also produce a wide range of fluorescently labeled streptavidins for immunochemistry.

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