Protein Biotinylation Kit
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| BB21-1rxn
/ for 100 ug IgG
|
1 rxn
|
$156.00
|
1 天 | |
| BB21-10rxn
/ for 10× 100 ug IgG
|
10 rxn
|
$389.00
|
21 天 |
Protein Biotinylation Kit is a ready-to-use kit for covalent labeling of purified proteins, peptides, and antibodies with biotin using activated NHS-ester biotin. The kit provides fast, reproducible, and gentle biotinylation of primary amino groups (the ε-amino groups of lysine residues and N-terminal amino groups) without significantly affecting the protein’s biological activity.
The optimized protocol enables biotinylation in a short time and allows control over the degree of labeling by adjusting the reagent/protein ratio. The resulting biotinylated conjugates are fully compatible with streptavidin-biotin systems and with standard detection and affinity-immobilization methods.
Key Features
- One-step biotinylation of primary amino groups using NHS-biotin
- Fast reaction at physiological and slightly alkaline pH
- Suitable for most purified peptides, proteins, and antibodies
- Does not require prior protein modification
- Preserves native protein properties due to mild reaction conditions
- Compatible with ELISA, Western blotting, enzyme immunoassay, immunochemistry, flow cytometry, and affinity methods
Applications
- Preparation of biotinylated antibodies and antigens
- Streptavidin/avidin-based immunoassays
- Affinity immobilization of proteins
- Cellular and biochemical research
- Development of diagnostic reagents
We also produce a wide range of fluorescently labeled streptavidins for immunochemistry.
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| 安全数据表（MSDS）: | 下载 |
| 储存时间（月: | 12 |
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