sulfo-Cyanine3 streptavidin
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| A13S0 | 100 ug | – | 现货 | |
| 113S0 | 1 mg | $185 | 现货 |
Streptavidin is a tetrameric biotin-binding protein derived from the bacterium Streptomyces avidinii. Streptavidin binds up to four biotin molecules with high affinity and selectivity via multiple hydrogen bonds and van der Waals interactions. Due to the lack of carbohydrate modifications and a near-neutral pI, streptavidin exhibits less nonspecific binding than another biotin-binding protein — avidin. Streptavidin also has high thermostability and resistance against extreme pH, denaturing agents, and enzymatic degradation, allowing using this protein under various experimental conditions.
Fluorescent conjugates of streptavidin are commonly used as a second-step reagent for specific detection of a variety of biotin-labeled biomolecules, such as proteins (antibodies, etc.), nucleic acids, lipids, and other molecules in indirect immunofluorescent staining, western blots, flow cytometry, microplate assays, and other detection techniques.
This streptavidin is a lyophilized conjugate with sulfo-Cyanine3, a hydrophilic orange fluorophore with spectral characteristics similar to Cy3® (absorption max. at 548 nm, emission max. at 563 nm).
The recommended concentration range for use is 0.5-10 µg/mL. Avoid using biotin-containing solutions (some serums, RPMI 1640, etc.) as diluents.
计算器
顾客还购买了这个产品
DMSO (dimethyl sulfoxide), labeling grade
Pure dimethyl sulfoxide (DMSO) solvent with quality optimized for bioconjugation reactions.AF 488 叠氮化物 (AF 488 azide)
AF 488 叠氮化物是一种发射在绿色光谱范围内的荧光团。它与炔烃在铜催化的条件下发生环加成反应。sulfo-Cyanine3-PEG3-biotin
Conjugate of hydrophilic sulfo-Cyanine3 dye with biotin, affinity label for avidin/streptavidin.General properties
| Appearance: | pink solid |
| Solubility: | good in water |
| Storage conditions: | Transportation: at room temperature for 1 week. Store at -20°С 9 months. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
您的购物车是空的
$ 
