AF 594 biocytin
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AF 594 biocytin is a cell-impermeant polar tracer. It consists of AF 594 dye, biotin, and aldehyde-fixable primary amine.
AF 594 is a bright, photostable red fluorophore with spectral characteristics similar to Texas Red (absorption max. at 586 nm, emission max. at 613 nm). Biotin allows the detection of the tracer with enzyme-containing streptavidin and subsequent amplification.
Polar tracers are water-soluble and can be introduced into cells by iontophoresis, patch clamping, osmotic lysis of pinocytic vesicles, and other comparable methods. AF 594 biocytin can be used to examine plasma membrane permeability, pinocytosis, transport through gap junctions, and cell-cell or cell-liposome fusion. It can also be used as a retrograde and anterograde neuronal tracer in live tissue.
Stock solution can be prepared in DMSO or DMF before use.
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Biocytin hydrochloride
Lysine derivative of biotin for conjugation with avidin or streptavidin, neuroanatomical tracer.Annexin V-AF 488 结合物 (Annexin V-AF 488 conjugate)
即用型试剂盒，可使用Annexin V-AF 488 结合物和碘化丙啶标记凋亡和坏死细胞。General properties
| Appearance: | blue solid |
| Molecular weight: | 1279.66 |
| Molecular formula: | C63H90N8O14S3 |
| Solubility: | soluble in water, DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 586 |
| ε, L⋅mol−1⋅cm−1: | 105000 |
| Emission maximum, nm: | 613 |
| Fluorescence quantum yield: | 0.77 |
| CF260: | 0.28 |
| CF280: | 0.51 |
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