Annexin V-AF 488 conjugate
目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
---|---|---|---|---|
11515 | 1 ug | – | 现货 | |
21515 | 5 ug | $119 | 现货 |
Annexin V (or Annexin A5) belongs to the phospholipid-binding Annexin family of intracellular proteins. In flow cytometry and fluorescent microscopy, Annexin V is commonly used to detect apoptotic cells by its ability to specifically bind to phosphatidylserine, which migrates from the inner to the outer leaflet of the plasma membrane during the early stages of apoptosis.
This Annexin V is a lyophilized conjugate with AF 488, a bright, photostable green fluorophore with spectral characteristics similar to FITC (absorption max. at 495 nm, emission max. at 519 nm).
Annexin V-AF 488 staining alone does not segregate the populations of apoptotic and necrotic cells. For this, additional staining with nuclear dyes that do not penetrate living cells — with propidium iodide or YODi-3 is required. For this purpose, you can also use our ready-to-use Annexin V-AF 488 apoptosis detection kit.
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(5,6)-FAM diacetate NHS ester
Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.AF 488 NHS 酯 (AF 488 NHS ester)
AF 488 NHS 酯,一种明亮、光稳定、亲水性的绿色通道荧光染料。Cyanine3 NHS ester minimal dye
Cyanine3 minimal dye for protein labeling.General properties
Appearance: | dark orange solid |
Solubility: | good in water |
Storage conditions: | Transportation: at room temperature for 1 week. Store at -20°С 9 months. |
安全数据表(MSDS): | 下载 |
产品规格 |
Spectral properties
Excitation/absorption maximum, nm: | 495 |
ε, L⋅mol−1⋅cm−1: | 71800 |
Emission maximum, nm: | 519 |
Fluorescence quantum yield: | 0.91 |
CF260: | 0.16 |
CF280: | 0.10 |
产品引用
- Fayzullina, D.; Yakushov, S.; Kantserova, K.; Belyaeva, E.; Aniskin, D.; Tsibulnikov, S.; Fayzullina, N.; Kalinin, S.; Romantsova, O.; Timashev, P.S.; Schroeder, B.A.; Ulasov, I.V. Carbonic Anhydrase Inhibitors Induce Ferroptosis through Inhibition of AKT/FTH1 Signaling in Ewing Sarcoma Tumor Cells. Cancers, 2023, 15(21), 5225. doi: 10.3390/cancers15215225