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Bis-ANS, protein conformation probe

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4381-5mg 5 mg $159 现货
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Bis-ANS is a high-affinity fluorescent probe for nonpolar cavities in proteins. Its hydrophobic phenyl and naphthyl rings interact noncovalently with proteins and protein degradation products.

As with other anilinonaphthalene sulfonates (ANS), bis-ANS is essentially nonfluorescent in water but becomes noticeably fluorescent in a nonpolar environment. When free, bis-ANS has an excitation maximum at 390 nm and an emission maximum at 523 nm but undergoes a blue shift with an increase in fluorescence intensity when bound to protein.

Bis-ANS is frequently used to monitor the formation of protein aggregates and indicate protein folding and conformational changes. The dye is also used to detect Aβ fibers.

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General properties

Appearance: light yellow-green crystals
Molecular weight: 672.87
CAS number: 65664-81-5
Molecular formula: C32H22K2N2O6S2
IUPAC name: dipotassium;8-anilino-5-(4-anilino-5-sulfonatonaphthalen-1-yl)naphthalene-1-sulfonate
Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, ethanol: slightly soluble, PBS (pH 7.2): 5 mg/ml
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
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