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sulfo-Cyanine5 tyramide

磺基-花菁5 酪酰胺显微镜观察

目录号	数量	价格	提前通知时间	购买此产品
3796-1mg	1 mg	$110	现货

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Tyramide signal amplification (TSA) is the most versatile and effective way to enhance the intensity of the fluorescent signal, used in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). The TSA method is based on the ability of horseradish peroxidase (HRP) in the presence of low concentrations of hydrogen peroxide to convert a labeled tyramine-containing substrate into an oxidized, highly reactive free radical that covalently binds to the tyrosine residues of protein molecules located next to it.

Compared to conventional procedures, the TSA method increases the sensitivity of immunofluorescent detection of target molecules by more than 100 times, making it particularly suitable for detecting low-concentration targets. In applications where increased detection sensitivity is not required, TSA can significantly reduce antibody or probe concentrations without loss of signal intensity, thereby reducing background staining due to cross-reactivity or non-specific binding of antibodies.

Since the binding of the tyramide label is covalent, tyramides of different dyes can be used in several sequential rounds of TSA staining to detect multiple targets in the same sample.

This tyramide is a conjugate of the water-soluble far-red fluorescent dye sulfo-Cyanine5. sulfo-Cyanine5 tyramide (also known as Cy5® and Cyanine5 tyramide from other manufacturers) is a component of many tyramide signal amplification (TSA) kits. It can be used with any antibody or other molecules (streptavidin, etc.) conjugated to HRP to stain cells and tissues by immunofluorescence methods.

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General properties

Appearance:	dark blue solid
Molecular weight:	800.05
Molecular formula:	C₄₀H₄₆KN₃O₈S₂
Solubility:	soluble in water, DMSO, DMF
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
安全数据表（MSDS）:	下载
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Spectral properties

Excitation/absorption maximum, nm:	646
ε, L⋅mol⁻¹⋅cm⁻¹:	271000
Emission maximum, nm:	662
Fluorescence quantum yield:	0.28
CF₂₆₀:	0.04
CF₂₈₀:	0.04

Cy® is a trademark of Cytiva.

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.