FAM-11-dUTP, 6-isomer
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2077-10nmol | 10 nmol | – | 现货 | |
| 2077-50nmol | 50 nmol | $190 | 现货 | |
| 2077-250nmol | 250 nmol | $325 | 现货 | |
| 2077-500nmol | 500 nmol |
$500
|
现货 | |
| 2077-1umol | 1 umol |
$910
|
现货 |
6-FAM-11-2’-deoxyuridine-5’-triphosphate, trilithium salt, is a common agent for non-radioactive DNA labeling.
FAM (fluorescein) is a popular green-fluorescent dye with an emission maximum at 513 nm. This derivative is a pure FAM 6-isomer.
6-FAM-11-dUTP is a modified triphosphate that can be incorporated into DNA instead of dTTP by various enzymes, including Klenow fragment, Taq-Polymerase, DNA-Polymerase I, Phi 29 Polymerase, reverse transcriptase or terminal transferase.
In contrast to triphosphates with a dye directly linked to aminoallyl-dUTP, this triphosphate contains a linker of 11 atoms between the fluorophore and the nitrogenous base. This linker length prevents FAM-label from potential static quenching and increases the efficiency of nucleotide incorporation during DNA synthesis.
6-FAM-11-dUTP can be used to produce a labeled product during PCR and cDNA synthesis and for labeled DNA-probes synthesis by random primed labeling and Nick-translation. Synthesized fluorescence-labeled DNA probes can be used to detect specific sequences by Southern blot, Northern blot, in situ hybridization, or by microarray analysis.
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抗坏血酸 (Ascorbic acid)
抗坏血酸是一种还原剂，可将铜 (II) 转化为用于催化点击反应的一价形式。FAM-dT亚磷酰胺
亚磷酰胺用于在合成序列中间，用荧光素 (FAM) 标记寡核苷酸。
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General properties
| Appearance: | yellow-greenish solid |
| Molecular weight: | 1013.49 |
| Molecular formula: | C39H39N4Li3O21P3 |
| IUPAC name: | ((2R,3S,5R)-5-(5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate |
| Solubility: | soluble in water |
| Quality control: | HPLC-MS (95%), testing in enzymatic reaction |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 492 |
| ε, L⋅mol−1⋅cm−1: | 74000 |
| Emission maximum, nm: | 517 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.22 |
| CF280: | 0.17 |
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