ProteOrange Protein Quantification Reagent, 500×
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 11210 | 100 uL | – | 现货 | |
| 41210 | 1 mL | $195 | 现货 |
ProteOrange is a fluorescent protein-sensitive dye that exhibits almost no fluorescence in the free form but fluoresces upon binding to proteins. The excitation maximum of ProteOrange in the complex with protein is at ~485 nm, the emission maximum — at ~590 nm.
The unique structure of the ProteOrange reagent allows accurate detection of proteins in solutions with concentrations between 10 ng/mL and 10 µg/mL. This level of sensitivity is considerably better than that achieved with the BCA method, Bradford assay, Lowry assay, or absorption measurements at 280 nm. The dye shows minimal variability for different proteins.
The ProteOrange dye has a low tolerance to non-ionic detergents. It is not recommended for cell lysates containing Triton X-100 and sodium deoxycholate, but it can tolerate up to 0.01% SDS (in final concentration).
This reagent is a 500× concentrate of ProteOrange dye. This product is a component of ProteOrange Protein Quantification Kit.
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dsSafe Nucleic Acid Gel Staining Solution, 10,000×
Fluorescent dye for DNA and RNA gel visualization, the best and safer alternative to ethidium bromide.ProteOrange Protein Gel Stain, 5000×
ProteOrange is a fluorescent dye for the staining of proteins in gels. ProteOrange is an analog of SYPRO orange. It is more sensitive than Coumassie.LumiTracker® Mito Green FM
Сell-permeant cationic green-fluorescent dye for staining active mitochondria in living cells in a potential-dependent manner.General properties
| Appearance: | Orange solution |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 470 |
| Emission maximum, nm: | 570 |
产品引用
- Kladova, O.A.; Tyugashev, T.E.; Yakimov, D.V.; Mikushina, E.S.; Novopashina, D.S.; Kuznetsov, N.A.; Kuznetsova, A.A. The Impact of SNP-Induced Amino Acid Substitutions L19P and G66R in the dRP-Lyase Domain of Human DNA Polymerase β on Enzyme Activities. International Journal of Molecular Sciences, 2024, 25(8), 4182. doi: 10.3390/ijms25084182
- Berditchevskii, G.M.; Vasina, L.V.; Ageev, S.V.; Meshcheriakov, A.A.; Galkin, M.A.; Ishmukhametov, R.R.; Nashchekin, A.V.; Kirilenko, D.A.; Petrov, A.V.; Martynova, S.D.; Semenov, K.N.; Sharoyko, V.V. A comprehensive study of biocompatibility of detonation nanodiamonds. Journal of Molecular Liquids, 2021, 332, 115763. doi: 10.1016/j.molliq.2021.115763
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