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ProbeMaster® Lyo GEL UDG, 5×

聚合酶链式反应和实时荧光定量PCR

目录号	数量	价格	提前通知时间	购买此产品
L0524	100 rxn	$119.00	20 天

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ProbeMaster^® Lyo GEL UDG is a lyophilized, ready-to-use reaction mixture containing all the necessary components for PCR with subsequent detection by electrophoresis. To reconstitute the mixture into a liquid form, add the specified amount of water. The mixture composition is optimized to obtain ideal results in terms of processivity and amplification specificity. Thanks to the high mixture density and the presence of dyes (Bromophenol Blue and Xylene Cyanol), the sample does not need to be mixed with loading buffer before application to the gel. The presence of two dyes allows for precise control of electrophoresis time.

The ProbeMaster^® Lyo GEL UDG reaction mixture is suitable for DNA amplification, with results subsequently detected by electrophoresis. Uracil-DNA glycosylase eliminates contamination from amplicons generated in previous reactions and prevents false-positive results, especially during electrophoresis. Due to the presence of dUTP, the mixture is not suitable for applications where the amplification products need to be used further. For such applications, we recommend using our ProbeMaster^®Lyo GEL reaction mixture.

Reaction mixture composition

HS Taq DNA polymerase;
Uracil-DNA glycosylase (UDG);
Deoxynucleoside triphosphate mixture (including dUTP);
PCR buffer (contains Mg²⁺);
Dyes for gel loading;
Cryoprotectants

Key characteristics

One tube of lyophilized mixture, after dilution in 450 μL of water, is sufficient for 100 reactions of 25 μL each.
The mixture is completely ready for use. To set up the reaction, only the DNA sample, primers, and water need to be added to the mixture, which significantly saves time. The ready-to-use reaction mixture format reduces the risk of sample contamination.
Uracil-DNA glycosylase removes amplicon contamination from previous reactions and prevents false-positive results, which is especially important when performing amplicon electrophoresis.
Suitable for PCR of fragments up to 3000 bp in length, with no more than 70% GC content, and not requiring high-precision amplification.
Genomic, viral, plasmid DNA, and other templates, as well as cDNA obtained by reverse transcription, can be used.
Contains a highly processive Hot-Start Taq polymerase, activated at 95 °C for 1 minute. The HS Taq DNA polymerase is a complex of monoclonal antibodies with the enzyme. Heating the sample in the first PCR cycle inactivates the antibodies in the complex and activates the enzyme. The "Hot-Start" technology prevents non-specific amplification and primer dimer formation.
The composition and density of the mixture are optimized for direct application of the sample to an agarose gel after amplification.
Due to the dyes included in the mixture, samples are easy to load onto an agarose gel. The presence of two dyes (Bromophenol Blue and Xylene Cyanol) allows for precise control of electrophoresis time.

Applications

PCR with detection of amplification products using gel electrophoresis, and PCR after reverse transcription.

Equipment compatibility

Compatible with any thermal cycler.

PCR reaction mixture selection table

Reaction mixtures for quantitative PCR (RT-PCR)
Name	dsGreen	Eva488	ROX	UDG, dUTP	Application
ProbeMaster^®️ Lyo UDG Cat.# •0514	—	—	—	✔	qPCR with DNA probes or intercalating dye
ProbeMaster^® Lyo ROX Cat.# •0114	—	—	✔	—
ProbeMaster^®️ Lyo Eva488 Cat.# •0614	—	✔	—	—
ProbeMaster^®️ Lyo Eva488 ROXCat.# •0714	—	✔	✔	—
ProbeMaster^® Lyo dsGreen Cat.# •0814	✔	—	—	—
Reaction mixture for standard PCR
ProbeMaster^® Lyo GEL Cat.# •0024	—	—	—	—	PCR followed by gel electrophoresis analysis, contains dye for application to gel
ProbeMaster^® Lyo GEL UDGCat.# •0524	—	—	—	✔
Universal reaction mixture
ProbeMaster^®️ Lyo UNI Cat.# •0534	—	—	—	—	qPCR with DNA probes/intercalating dye or standard PCR followed by gel electrophoresis analysis

计算器

溶液计算器

General properties

Appearance:	deep blue tablet
Solubility:	water
Quality control:	functional test
Storage conditions:	Storage: 12 months (from the date of delivery) at 4 °C. Transportation: up to 21 days at a temperature of up to 25 °C. After reconstitution into liquid form, store at 4 °C for up to 30 days or freeze and store at -20 °C within the expiration date. The reconstituted mixture may undergo up to five freeze–thaw cycles.
安全数据表（MSDS）:	下载
	产品规格

ProbeMaster® is a registered trademark of Lumiprobe.

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.