ProteOrange Protein Gel Stain, 5000×
目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
---|---|---|---|---|
10210 | 100 uL | – | 现货 | |
40210 | 1 mL | $285 | 现货 |
ProteOrange is a protein-binding dye for staining of protein gels, an analog of SYPRO Orange. The fluorophore is a styrene-like zwitterionic dye. It binds proteins selectively in the presence of nucleic acids, polysaccharides, and other molecules. It is also tolerant to SDS, and therefore can be used for SDS-PAGE gel staining. The dye interacts with the SDS coat around proteins in the gel, so it gives more consistent staining between different types of proteins compared to CBB staining, and never exhibits negative staining. It also stains glycoproteins well.
Staining with ProteOrange is about an order of magnitude more sensitive than Coumassie dye staining, and allows to detect about 3 ng of protein per band (30 ng is detection limit for Coumassie).
ProteOrange is supplied as a 5000x solution in DMSO. 1 mL of the solution is enough for the staining of around 200 minigels.
![](https://static.lumiprobe.us/img/spectra/ProteOrange_excitation_emission_spectrum.png)
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胺反应性 Cyanine5 活化酯,用于标记蛋白质、肽和其他分子。Cyanine2 NHS ester minimal dye
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用于实时定量PCR 的 dsGreen 储备液,100×General properties
Appearance: | orange solution |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
安全数据表(MSDS): | 下载 |
产品规格 |
Spectral properties
Excitation/absorption maximum, nm: | 470 |
Emission maximum, nm: | 570 |
产品引用
- Heesink, G.; van den Oetelaar, M.C.M.; Semerdzhiev, S.A.; Ottmann, C.; Brunsveld, L.; Blum, C.; Claessens, M.M.A.E. 14-3-3τ as a Modulator of Early α-Synuclein Multimerization and Amyloid Formation. ACS Chemical Neuroscience, 2024, 15(9), 1926-1936. doi: 10.1021/acschemneuro.4c00100
- Somsen, B. A.; Sijbesma, E.; Leysen, S.; Honzejkova, K.; Visser, E. J.; Cossar, P. J.; Obšil, T.; Brunsveld, L.; Ottmann, C. Molecular Basis and Dual Ligand Regulation of Tetrameric Estrogen Receptor α/14-3-3ζ Protein Complex. Journal of Biological Chemistry, 2023, 299(7), 104855. doi: 10.1016/j.jbc.2023.104855
- Vasilev, R.; Gunitseva, N.; Shebanova, R.; Korzhenkov, A.; Vlaskina, A.; Evteeva, M.; Polushkina, I.; Nikitchina, N.; Toshchakov, S.; Kamenski, P.; Patrushev, M.; Mazunin, I. Targeted Modification of Mammalian DNA by a Novel Type V Cas12a Endonuclease from Ruminococcus bromii. International Journal of Molecular Sciences, 2022, 23(16), 9289. doi: 10.3390/ijms23169289
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