sulfo-Cyanine5 hydrazide
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 13370 | 1 mg | $125 | 现货 | |
| 23370 | 5 mg | $325 | 现货 | |
| 43370 | 25 mg |
$850
|
现货 | |
| 53370 | 50 mg |
$1490
|
现货 | |
| 63370 | 100 mg |
$1990
|
现货 |
sulfo-Cyanine5 is a sulfated derivative of Cyanine5 dye, well soluble in water because of two negatively charged sulfo groups in its structure. By its spectral characteristics, this far-red fluorescent dye is an analog of Cy™5.
Hydrazides efficiently react with aldehydes and ketones resulting in hydrazones, so this compound can be used for conjugation with carbonyl derivatives of biomolecules.
The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with sulfo-Cyanine5 hydrazide). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can be also conjugated with sulfo-Cyanine5 hydrazide in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.
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磺基-Cyanine3酰肼 (sulfo-Cyanine3 hydrazide)
具有磺基的Cyanine3酰肼，易溶于水，易与羰基化合物反应。FAM 酰肼，5-异构体 (FAM hydrazide, 5-isomer)
FAM 酰肼是一种羰基反应性荧光素衍生物。纯5-异构体。可以用羰基的氧化蛋白质、糖类和许多小分子。炔酰肼 (Alkyne hydrazide)
含有炔基和酰肼官能团的双功能试剂。它与醛和酮发生反应。然后炔基可以通过 CuAAC 反应与各种叠氮化物偶联。General properties
| Appearance: | dark blue solid |
| Molecular weight: | 656.83 |
| CAS number: | 2055138-61-7 |
| Molecular formula: | C32H40N4O7S2 |
| Solubility: | very good in water, good in DMF and DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
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