sulfo-Cyanine5 hydrazide
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 13370 | 1 mg | $125 | 现货 | |
| 23370 | 5 mg | $325 | 现货 | |
| 43370 | 25 mg |
$850
|
现货 | |
| 53370 | 50 mg |
$1490
|
现货 | |
| 63370 | 100 mg |
$1990
|
现货 |
sulfo-Cyanine5 is a sulfated derivative of Cyanine5 dye, well soluble in water because of two negatively charged sulfo groups in its structure. By its spectral characteristics, this far-red fluorescent dye is an analog of Cy™5.
Hydrazides efficiently react with aldehydes and ketones resulting in hydrazones, so this compound can be used for conjugation with carbonyl derivatives of biomolecules.
The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with sulfo-Cyanine5 hydrazide). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can be also conjugated with sulfo-Cyanine5 hydrazide in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.
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磺基-Cyanine5马来酰亚胺
适用于 Cy5® 通道的硫基反应性水溶性花青染料。sulfo-Cyanine3 NHS酯 (sulfo-Cyanine3 NHS ester)
水溶性磺基-Cyanine3琥珀酰亚胺酯 (SE) 活化酯，用于氨基生物分子标记。磺基-Cyanine3马来酰亚胺
磺基-Cyanine3的水溶性硫醇反应衍生物，一种Cy3 类似物。General properties
| Appearance: | dark blue solid |
| Molecular weight: | 656.83 |
| CAS number: | 2055138-61-7 |
| Molecular formula: | C32H40N4O7S2 |
| Solubility: | very good in water, good in DMF and DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
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