dsGreen 用于实时定量PCR, 100× (dsGreen for Real-Time PCR, 100×)

目录号 数量 价格 提前通知时间
A1010 50 uL –   现货
11010 100 uL $39 现货
41010 1 mL $235 现货
71010 5 mL $510 现货
91010 50 mL $2670 现货
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dsGreen 是一种非常灵敏的双链 DNA 检测染料。 dsGreen 的高灵敏度和高选择性使得dsGreen可作为 qPCR 的通用 dsDNA 检测试剂。用dsGreen染料,无需使用标记探针来检测扩增,用未标记引物即可。

与 Lumiprobe公司提供的用于凝胶染色的 dsGreen 其他制剂不同,该制剂专门设计用于实时荧光定量PCR 实验。具体特征为:

  • 染料浓度针对 qPCR 进行了优化,并经过仔细调整,以获得批次之间的可重复结果
  • PCR 测试制剂 – 质量有保证
  • 低荧光背景-高荧光强度增益
  • 推荐协议

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    外观: 浅橙色溶液
    质量控制: NMR 1H、HPLC-MS (95%)、PCR 检测
    储存条件: 储存:收到后-20℃避光保存24个月。 运输:室温下最多可保存3周。 避免长时间暴露在光线下。
    安全数据表(MSDS): 下载
    产品规格

    激发/吸收极大值,纳米: 490
    ε, 摩尔吸光系数,cm⁻¹: 73000
    发射极大值,纳米: 524
    荧光量子产率: 0,8

    产品引用

    1. Bykonia, E.N.; Kleymenov, D.A.; Gushchin, V.A.; Sinyavin, A.E.; Mazunina, E.P.; Kuznetsova, N.A.; Kozlova, S.R.; Zolotar, A.N.; Shidlovskaya, E.V.; Usachev, E.V.; Pochtovyi, A.A.; Kustova, D.D.; Ivanov, I.A.; Dmitriev, S.E.; Ivanov, R.A.; Logunov, D.Y.; Gintsburg, A.L. Major role of S-glycoprotein in providing immunogenicity and protective immunity in mRNA lipid nanoparticle vaccines based on SARS-CoV-2 structural proteins. bioRxiv, 2024, preprint. doi: 10.1101/2023.12.30.573713
    2. Huber, R.; Lee, J.; Borretta, L.; Tessier-Cloutier, B.; Lum, A.; Yip, S.; Horst, B.A. TERT promoter mutations in atypical melanocytic lesions: A series of seven cases with adverse melanoma-specific outcome. Human pathology, 2024, 144, 34-39. doi: 10.1016/j.humpath.2024.01.003
    3. Sakhabutdinova, A. R.; Chemeris, A. V.; Garafutdinov, R. R. Detection of Specific RNA Targets by Multimerization. Biochemistry (Moscow), 2023, 88(5), 679–686. doi: 10.1134/S0006297923050103
    4. Hildebrandt, R.P.; Moss, K.R.; Janusz-Kaminska, A.; Knudson, L.A.; Denes, L.T.; Saxena, T.; Boggupalli, D.P.; Li, Z.; Lin, K.; Bassell, G.J.; Wang, E.T. Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes. Nature Communications, 2023, 14, 3427. doi: 10.1038/s41467-023-38923-6
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