DAPI, blue fluorescent nucleic acid stain
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2996-1mL | 1 mL, 1 mg/mL in Water | $75 | 现货 | |
| 2996-50uL | 50 uL, 1 mg/mL in Water | – | 现货 | |
| 2996-1mg | 1 mg | $30 | 现货 | |
| 2996-10mg | 10 mg | $110 | 现货 |
DAPI (4′,6-diamidino-2-phenylindole) is a blue-emitting fluorescent dye that binds strongly to adenine-thymine-rich regions in DNA. It is used extensively in fluorescence microscopy, flow cytometry for chromosome staining and as a nuclear counterstain. DAPI is generally used to stain fixed cells since the dye is membrane impermeant, although the dye can enter live cells when used at high concentrations.
When bound to double-stranded DNA, DAPI exhibits ~20-fold enhancement of fluorescence with an absorption maximum at 358 nm and emission maximum at 461 nm. DAPI also binds to RNA, though it is not as strongly fluorescent. When bound to RNA, its emission spectrum shifts to around 500 nm.
There is a slight fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and GFP. Use spectral unmixing if extremely precise image analysis is required.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting DNA and detecting annealed probes in blotting applications by specifically visualizing the double-stranded complex. Also, DAPI staining is a sensitive and specific detection method for mycoplasma.
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DusQ 2 phosphoramidite
DusQ 2 phosphoramidite for the synthesis of oligonucleotides labeled with DusQ 2 quencher at 3'-end, 5'-end and internal position. The phosphoramidite has DMT protection and can be used for cartridge purification.
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Cyanine3 phosphoramidite
Phosphoramidite for the synthesis of 5’-Cyanine3-labeled oligonucleotides.TODi-1，绿色荧光核酸染料 (TODi-1, green fluorescent nucleic acid stain)
TODi-1是一种细胞不可渗透性的绿色荧光核酸染料。TODi-1 的化学性质与TOTO®-1相同。General properties
| Appearance: | yellow solid / solution |
| Molecular weight: | 350.25 |
| CAS number: | 28718-90-3 |
| Molecular formula: | C16H17Cl2N5 |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 355 |
| ε, L⋅mol−1⋅cm−1: | 28800 |
| Emission maximum, nm: | 460 |
产品引用
- Potapenko, E.Y..; Kashko, N.D.; Knorre, D.A. Spontaneous Mutations in Saccharomyces cerevisiae mtDNA Increase Cell-to-Cell Variation in mtDNA Amount. International Journal of Molecular Sciences, 2023, 24(24), 17413. doi: 10.3390/ijms242417413
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