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DAPI, blue fluorescent nucleic acid stain

细胞活力和细胞凋亡细胞核显微镜观察流式细胞术

目录号	数量	价格	提前通知时间
2996-1mL	1 mL, 1 mg/mL in Water	$75	20 天 (起定量:2 个起)
2996-50uL	50 uL, 1 mg/mL in Water	–	现货
2996-1mg	1 mg	$30	现货 (起定量:3 个起)
2996-10mg	10 mg	$110	现货

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DAPI (4′,6-diamidino-2-phenylindole) is a blue-emitting fluorescent dye that binds strongly to adenine-thymine-rich regions in DNA. It is used extensively in fluorescence microscopy, flow cytometry for chromosome staining and as a nuclear counterstain. DAPI is generally used to stain fixed cells since the dye is membrane impermeant, although the dye can enter live cells when used at high concentrations.

When bound to double-stranded DNA, DAPI exhibits ~20-fold enhancement of fluorescence with an absorption maximum at 355 nm and emission maximum at 460 nm. DAPI also binds to RNA, though it is not as strongly fluorescent. When bound to RNA, its emission spectrum shifts to around 500 nm.

There is a slight fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and GFP. Use spectral unmixing if extremely precise image analysis is required.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting DNA and detecting annealed probes in blotting applications by specifically visualizing the double-stranded complex. Also, DAPI staining is a sensitive and specific detection method for mycoplasma.

The working concentration of DAPI for staining eukaryotic cells is usually 1 μg/mL. Still, it depends on the cell type and density, so the exact dilution of the dye should be determined experimentally.

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Staining cells with Lumiprobe's DAPI dye

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General properties

Appearance:	yellow solid / solution
Molecular weight:	350.25
CAS number:	28718-90-3
Molecular formula:	C₁₆H₁₇Cl₂N₅
Solubility:	good in DMF, DMSO
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
安全数据表（MSDS）:	下载
	产品规格

Spectral properties

Excitation/absorption maximum, nm:	355
ε, L⋅mol⁻¹⋅cm⁻¹:	28800
Emission maximum, nm:	460

产品引用

Samatoshenkov, I.V.; Aimaletdinov, A.M.; Zakirova, E.Y.; Chelyshev, Y.A.; Samatoshenkova, J.M.; Kadyrov, M.S.; Kniazev, E.A.; Salakhov, B.I.; Mukhamedshina, Y.O. The Combined Delivery of the Vegf, Ang, and Gdnf Genes Stimulates Angiogenesis and Improves Post-Ischemic Innervation and Regeneration in Skeletal Muscle. Current Issues in Molecular Biology, 2024, 46(8), 8611-8626. doi: 10.3390/cimb46080507
Potapenko, E.Y..; Kashko, N.D.; Knorre, D.A. Spontaneous Mutations in Saccharomyces cerevisiae mtDNA Increase Cell-to-Cell Variation in mtDNA Amount. International Journal of Molecular Sciences, 2023, 24(24), 17413. doi: 10.3390/ijms242417413

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.