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TAMRA CPG 1000, 6-isomer

目录号 数量 价格 提前通知时间
17760 100 mg
$95.00
现货
47760 1 g
$469.00
现货
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6-TAMRA CPG (glycerol) is controlled pore glass (CPG) with the fluorescent dye 6-carboxytetramethylrhodamine (6-TAMRA) covalently attached via a glycerol linker. This support is designed for automated solid-phase oligonucleotide synthesis using the phosphoramidite method, allowing the introduction of a fluorescent label at the 3′-end without additional post-synthetic labeling steps.

The 1000 Å pore size is optimal for synthesizing long sequences — up to 100 nucleotides. 6-TAMRA is a orange-red fluorescent dye with absorption and emission maxima at 541 nm and 567 nm, respectively, and is widely used in molecular diagnostics. TAMRA-labeled oligonucleotides obtained with this reagent are employed in methods such as real-time PCR, fluorescence in situ hybridization (FISH), FRET, and surface-enhanced Raman spectroscopy (SERS).

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General properties

Appearance: deep pink beads
Quality control: NMR 1H and loading measurement, functional testing in oligo synthesis.
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
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产品规格

Spectral properties

Excitation/absorption maximum, nm: 541
ε, L⋅mol−1⋅cm−1: 84000
Emission maximum, nm: 567
Fluorescence quantum yield: 0.1
CF260: 0.32
CF280: 0.19

Oligo synthesis details

Pore size, Å: 1000
Typical loading, umol/g: 30-40
Coupling conditions: standard time depending on the first monomer
Cleavage conditions: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 h at 60 °C, then cool down to room temperature.
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