TAMRA phosphoramidite, 5-isomer
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2497-100mg | 100 mg | 请查询 | 现货 (3 pcs) | |
| 2497-250mg | 250 mg | 请查询 | 现货 (2 pcs) | |
| 2497-1g | 1 g | 请查询 | 现货 (2 pcs) | |
| 2497-5g | 5 g | 请查询 | 现货 (2 pcs) |
This phosphoramidite is used for synthesis of oligonucleotides 5’-labeled with TAMRA.
TAMRA (carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor (and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which contain 5’-terminal TAMRA and FAM in the middle of the sequence or at the 3’-end (using Fluorescein dT Phosphoramidite and FAM CPG, respectively).
TAMRA 5’-labeled oligonucleotides are commonly used for quantitative PCR and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection channel for TAMRA frequently.
The TAMRA dye is not stable in the presence of ammonium and sterically non-hindered primary amines, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.
Usage
Coupling: 7.5 min.
Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 hours at 60 °C, then cool down to room temperature.
Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support.
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DusQ1 dT 亚磷酰胺 (DusQ 1 dT phosphoramidite)
用于寡核苷酸DusQ1 dT 标记的亚磷酰胺。
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炔基亚磷酰胺，5'-端 (Alkyne phosphoramidite, 5'-terminal)
炔基烃亚磷酰胺，用于合成可用于点击化学的寡核苷酸。General properties
| Appearance: | purple solid |
| Mass spec M+ increment: | 589.60 |
| Molecular weight: | 727.83 |
| Molecular formula: | C40H50N5O6P |
| Solubility: | good in acetonitrile, dichloromethane |
| Quality control: | NMR 1H, HPLC-MS (95%), coupling test |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 541 |
| ε, L⋅mol−1⋅cm−1: | 84000 |
| Emission maximum, nm: | 567 |
| CF260: | 0.32 |
| CF280: | 0.19 |
Oligo synthesis details
| Diluent: | acetonitrile |
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