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TET azide, 6-isomer

目录号 数量 价格 提前通知时间
18230 1 mg $110 现货
28230 5 mg $165 现货
48230 25 mg $285 现货
58230 50 mg $495 现货
68230 100 mg $880 现货
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TET (tetrachlorofluorescein) is a green-fluorescent fluorescein derivate with absorption maximum at 519 nm and emission maximum at 535 nm. TET is spectrally similar to R6G, JOE, and VIC, widely used for labeling PCR probes.

Oligonucleotides labeled with TET are often used in real-time PCR; the preparation of such oligonucleotides can be performed using click chemistry. This derivative is an azide, a pure 6-isomer, for conjugating TET to other molecules by copper-catalyzed and copper-free click reactions.

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R6G azide, 5-isomer

Rhodamine 6G is a xanthene dye that has been used for DNA sequencing and qPCR. This azide derivative is used for the attachment of the dye to DNA and other molecules by means of click chemistry.

VIC azide, 6-isomer

VIC is an asymmetric xanthene dye with spectral properties similar to HEX and JOE, widely used for labeling PCR probes. This derivative is an azide for dye conjugation by click chemistry.

General properties

Appearance: orange powder
Molecular weight: 596.21
CAS number: 1450752-90-5
Molecular formula: C24H14Cl4N4O6
Solubility: in DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
安全数据表(MSDS): 下载
产品规格

Spectral properties

Excitation/absorption maximum, nm: 519
ε, L⋅mol−1⋅cm−1: 100000
Emission maximum, nm: 535
Fluorescence quantum yield: 0.47
CF260: 0.17
CF280: 0.09
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