LumiTracker® Mito TMRE
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2274-1mg | 1 mg | – | 现货 | |
| 2274-25mg | 25 mg | $190 | 现货 |
TMRE is widely used for labeling mitochondria in live cells but not compatible with fixation. This lipophilic and positively charged dye rapidly permeates plasma membrane without interacting with membrane proteins and forming aggregates. TMRE selectively accumulates in active mitochondria due to their transmembrane potential.
In addition to staining mitochondria for imaging purposes, TMRE is used for quantitative measurements of mitochondria membrane potential using Nernst equation. The dye serves as a tool to study mitochondrial function changes and cell viability in response to stimuli or pharmaceuticals of interest. Mitochondrial depolarization caused by apoptosis, necrosis or other factors is characterized by decreased membrane potential and is indicated with decreased fluorescence compared to intact cells that have polarized mitochondria.
TMRE applications include fluorescent microscopy, flow cytometry, microplate assays. The dye has an excitation maximum at 549 nm: it can be effectively excited by the blue (488 nm) or yellow-green (561 nm) lasers. Emission of the dye can be detected in PE channel (maximum at 574 nm).
顾客还购买了这个产品
BDP® TR ceramide
Fluorescent derivative of sphingosine with emission in the red spectrum region for visualization of the Golgi apparatus in cells.AF 488 叠氮化物
AF 488 叠氮化物是一种发射在绿色光谱范围内的荧光团。它与炔烃在铜催化的条件下发生环加成反应。BDP® TMR ceramide
Fluorescent derivative of sphingosine with emission in the orange spectrum region for visualization of the Golgi apparatus in cells.General properties
| Appearance: | dark colored solid |
| Molecular weight: | 514.96 |
| CAS number: | 115532-52-0 |
| Molecular formula: | C26H27N2ClO7 |
| IUPAC name: | 3,6-bis(dimethylamino)-9-(2-ethoxycarbonylphenyl)xanthylium perchlorate |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| ε, L⋅mol−1⋅cm−1: | 156000 |
| Emission maximum, nm: | 575 |
| Fluorescence quantum yield: | 0.66 |
产品引用
- Moiseeva, N.; Eroshenko, D.; Laletina, L.; Rybalkina, E.; Susova, O.; Karamysheva, A.; Tolmacheva, I.; Nazarov, M.; Grishko, V. The Molecular Mechanisms of Oleanane Aldehyde-β-Enone Cytotoxicity against Doxorubicin-Resistant Cancer Cells. Biology, 2023, 12(3), 415. doi: 10.3390/biology12030415
- Kozlova, T.; Popov, A.; Romanov, M. V.; Savintseva, I.; Vasilyeva, D. N.; Baranchikov, A.; Ivanov, V. Ceric Phosphates and Nanocrystalline Ceria: Selective Toxicity to Melanoma Cells. Nanosystems: Physics, Chemistry, Mathematics, 2023, 14, 223–230. doi: 10.17586/2220-8054-2023-14-2-223-230
- Tolmacheva, I.; Beloglazova, Y.; Nazarov, M.; Gagarskikh, O.; Grishko, V. Synthesis and Anticancer Activity of A-Ring-Modified Derivatives of Dihydrobetulin. International Journal of Molecular Sciences, 2023, 24(12), 9863. doi: 10.3390/ijms24129863
您的购物车是空的
$ 
