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Universal CPG type I, 500A
此产品已停产，不再提供. 但我们有现货（请参阅下文）
我们建议使用Universal CPG type II, 500A作为替代品
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 2646-1g | 1 g | 请查询 | 现货 (1 pcs) |
The Universal CPG type I, 500A is one of universal supports used to immobilize nucleosides for high-throughput synthesizing oligonucleotides.
The cleavage from the support is followed by deprotection using anhydrous methylamine gas, ammonium hydroxide/methylamine mixture and other basic reagents. Universal support CPG type I is suitable for relatively aggressive environment treatment. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases.
Usage
Coupling: Standard conditions for universal CPG.
Deprotection: 3 hours at 80 °C using AMA mixture, ammonium hydroxide / 40% methylamine (1:1) for a 50 nmol scale synthesis. The bigger scale of synthesis requires more than 4 hours to complete.
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通用 CPG II 型，1000A (Universal CPG type II, 1000A)
这种通用支持物旨在用于寡核苷酸的自动合成，以加速解封闭过程中的去磷酸化过程。对于多达 120 个碱基的寡核苷酸合成，建议使用 1000 Å 的孔径。DusQ 2 phosphoramidite
DusQ 2 phosphoramidite for the synthesis of oligonucleotides labeled with DusQ 2 quencher at 3'-end, 5'-end and internal position. The phosphoramidite has DMT protection and can be used for cartridge purification.
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DusQ 2 CPG 500
Controlled pore glass (CPG) 500 modified support for the synthesis of oligonucleotides with DusQ 2 quencher at the 3’ end. Compatible with standard deblocking conditions.
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General properties
| Appearance: | white to cream beads |
| Quality control: | loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at −20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−80 |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | AMA mixture, ammonium hydroxide - 40% methylamine (1:1), 3 hours at 80 °C |
| Deprotection conditions: | identical to protected nucleobases |
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