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VIC phosphoramidite, 6-isomer
目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
---|---|---|---|---|
BH260 | 50 mg | – | 现货 | |
DH260 | 250 mg | $490 | 现货 | |
FH260 | 1 g | $990 | 现货 |
VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.
This phosphoramidite reagent allows to introduce the label onto 5'-terminus of the oligonucleotide.
Recommendations for using the reagent:
Coupling: 10 minutes.
Deprotection: standard conditions with ammonium hydroxide; AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) (15 minutes at 65°C) can be used with minor side product forming.
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DusQ 2 dT 亚磷酰胺 (DusQ 2 dT phosphoramidite)
用于寡核苷酸 DusQ 2 dT 标记的亚磷酰胺。
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ROX azide, 6-isomer
Azide derivative of ROX (Rhodamine X, Rhodamine 101) dye for biomolecule labeling via click chemistry. Pure 6-isomer.ATT 550 叠氮化物 (ATT 550 azide)
水溶性黄色荧光染料 ATT 550 的叠氮衍生物,可用于含炔生物分子的标记和生物物体的荧光可视化。General properties
Appearance: | white / off white solid |
Molecular weight: | 1023.39 |
CAS number: | 1414265-81-8 |
Molecular formula: | C52H59Cl3N3O10P |
Quality control: | NMR 1H, 31P and HPLC-MS (95+%) |
Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
安全数据表(MSDS): | 下载 |
产品规格 |
Spectral properties
Excitation/absorption maximum, nm: | 525 |
ε, L⋅mol−1⋅cm−1: | 103000 |
Emission maximum, nm: | 546 |
Fluorescence quantum yield: | 0.53 |
CF260: | 0.07 |
CF280: | 0.07 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | standard coupling, identical to normal nucleobases |
Deprotection conditions: | standard deprotection conditions |
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