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VIC phosphoramidite, 6-isomer

目录号 数量 价格 提前通知时间
BH260 50 mg –   现货
DH260 250 mg $490 现货
FH260 1 g $990 现货
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VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.

This phosphoramidite reagent allows to introduce the label onto 5'-terminus of the oligonucleotide.

Recommendations for using the reagent:

Coupling: 10 minutes.

Deprotection: standard conditions with ammonium hydroxide; AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) (15 minutes at 65°C) can be used with minor side product forming.

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PreciseGreen dsDNA 定量试剂,200× (PreciseGreen dsDNA Quantification Reagent, 200×)

PreciseGreen 是一种染料,可用于在RNA、ssDNA 和其他杂质存在的情况下对dsDNA进行精确荧光定量。该染料在荧光素通道中表现出明亮的荧光,在pg/mLng/mL 范围内对 DNA 浓度提供线性响应。

DusQ 2 CPG 500

Controlled pore glass (CPG) 500 modified support for the synthesis of oligonucleotides with DusQ 2 quencher at the 3’ end. Compatible with standard deblocking conditions.
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磺基-Cyanine7 羧酸 (sulfo-Cyanine7 carboxylic acid)

sulfo-Cyanine7 carbacid 是一种非反应性荧光染料,可用作 NIR(近红外)标记物。

General properties

Appearance: white / off white solid
Molecular weight: 1023.39
CAS number: 1414265-81-8
Molecular formula: C52H59Cl3N3O10P
Quality control: NMR 1H, 31P and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
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Spectral properties

Excitation/absorption maximum, nm: 525
ε, L⋅mol−1⋅cm−1: 103000
Emission maximum, nm: 546
Fluorescence quantum yield: 0.53
CF260: 0.07
CF280: 0.07

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Deprotection conditions: standard deprotection conditions
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