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VIC phosphoramidite, 6-isomer
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| BH260 | 50 mg | – | 现货 | |
| DH260 | 250 mg |
$490
|
现货 | |
| FH260 | 1 g |
$990
|
现货 |
VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.
This phosphoramidite reagent allows to introduce the label onto 5'-terminus of the oligonucleotide.
Recommendations for using the reagent:
Coupling: 10 minutes.
Deprotection: standard conditions with ammonium hydroxide; AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) (15 minutes at 65°C) can be used with minor side product forming.
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Cyanine5亚磷酰胺 (Cyanine5 phosphoramidite)
Cyanine5 亚磷酰胺衍生物，用于寡核苷酸合成JOE phosphoramidite, 5-isomer
JOE phosphoramidite is a xanthene dye phosphoramidite used in oligo synthesis. Its absorption and emission spectra are located between the FAM and TAMRA channels.ATT 565 叠氮化物 (ATT 565 azide)
水溶性橙色荧光染料 ATT 565 的叠氮衍生物，用于标记含炔基的生物分子和生物体的荧光可视化。纯6-异构体。General properties
| Appearance: | white / off white solid |
| Molecular weight: | 1023.39 |
| CAS number: | 1414265-81-8 |
| Molecular formula: | C52H59Cl3N3O10P |
| Quality control: | NMR 1H, 31P and HPLC-MS (95+%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 525 |
| ε, L⋅mol−1⋅cm−1: | 103000 |
| Emission maximum, nm: | 546 |
| Fluorescence quantum yield: | 0.53 |
| CF260: | 0.07 |
| CF280: | 0.07 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Deprotection conditions: | standard deprotection conditions |
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