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VIC phosphoramidite, 6-isomer
目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
---|---|---|---|---|
BH260 | 50 mg | – | 现货 | |
DH260 | 250 mg | $490 | 现货 | |
FH260 | 1 g | $990 | 现货 |
VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.
This phosphoramidite reagent allows to introduce the label onto 5'-terminus of the oligonucleotide.
Recommendations for using the reagent:
Coupling: 10 minutes.
Deprotection: standard conditions with ammonium hydroxide; AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) (15 minutes at 65°C) can be used with minor side product forming.
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Cyanine5亚磷酰胺 (Cyanine5 phosphoramidite)
Cyanine5 亚磷酰胺衍生物,用于寡核苷酸合成
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Cyanine5.5 amine
Amino derivative of Cyanine5.5, a far red emitting fluorescent cyanine dye.
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ROX NHS 酯,6-异构体
ROX(罗丹明X,R101)NHS-酯的纯 6-异构体,用于胺标记。General properties
Appearance: | white / off white solid |
Molecular weight: | 1023.39 |
CAS number: | 1414265-81-8 |
Molecular formula: | C52H59Cl3N3O10P |
Quality control: | NMR 1H, 31P and HPLC-MS (95+%) |
Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
安全数据表(MSDS): | 下载 |
产品规格 |
Spectral properties
Excitation/absorption maximum, nm: | 525 |
ε, L⋅mol−1⋅cm−1: | 103000 |
Emission maximum, nm: | 546 |
Fluorescence quantum yield: | 0.53 |
CF260: | 0.07 |
CF280: | 0.07 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | standard coupling, identical to normal nucleobases |
Deprotection conditions: | standard deprotection conditions |
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