此页面上的某些内容不提供英文版本，默认显示为当前可用的语言。

AF 488 tyramide

AF 488 Tyramides Microscopy
目录号 数量 价格 提前通知时间
3805-1mg 1 mg $150 现货
批量询价
Found better price? Let us know and we will propose the way forward!

Thyramide signal amplification (TSA) is the most versatile and effective way to enhance the intensity of the fluorescent signal, used in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). The TSA method is based on the ability of horseradish peroxidase (HRP) in the presence of low concentrations of hydrogen peroxide to convert a labeled tyramine-containing substrate into an oxidized, highly reactive free radical that covalently binds to the tyrosine residues of protein molecules located next to it.

Compared to conventional procedures, the TSA method increases the sensitivity of immunofluorescent detection of target molecules by more than 100 times, making it particularly suitable for detecting low-concentration targets. In applications where increased detection sensitivity is not required, TSA can significantly reduce antibody or probe concentrations without loss of signal intensity, thereby reducing background staining due to cross-reactivity or non-specific binding of antibodies.

Since the binding of the tyramide label is covalent, tyramides of different dyes can be used in several sequential rounds of TSA staining to detect multiple targets in the same sample.

This tyramide is a conjugate of the water-soluble green fluorescent dye AF 488. AF 488 tyramide is a component of many tyramide signal amplification (TSA) kits. It can be used with any antibody or other molecules (streptavidin, etc.) conjugated to HRP to stain cells and tissues by immunofluorescence methods.

以ChemDraw格式下载

推荐协议

计算器

顾客还购买了这个产品

Biotin-11-UTP

Biotinylated UTP for enzymatic RNA labeling.

DMSO (dimethyl sulfoxide), labeling grade

Pure dimethyl sulfoxide (DMSO) solvent with quality optimized for bioconjugation reactions.

BDP® FL ceramide

Fluorescent derivative of sphingosine with emission in the green spectrum region for visualization of the Golgi apparatus in cells.

General properties

Appearance: orange solid
Molecular weight: 856.03
Molecular formula: C41H53N5O11S2
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
安全数据表（MSDS）: 下载
产品规格

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
您的商品已添加. 查看您的 购物车 或者 继续结账
商品数量不正确.