AF 488 tyramide
目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
---|---|---|---|---|
3805-1mg | 1 mg | $150 | 现货 |
Tyramide signal amplification (TSA) is the most versatile and effective way to enhance the intensity of the fluorescent signal, used in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). The TSA method is based on the ability of horseradish peroxidase (HRP) in the presence of low concentrations of hydrogen peroxide to convert a labeled tyramine-containing substrate into an oxidized, highly reactive free radical that covalently binds to the tyrosine residues of protein molecules located next to it.
Compared to conventional procedures, the TSA method increases the sensitivity of immunofluorescent detection of target molecules by more than 100 times, making it particularly suitable for detecting low-concentration targets. In applications where increased detection sensitivity is not required, TSA can significantly reduce antibody or probe concentrations without loss of signal intensity, thereby reducing background staining due to cross-reactivity or non-specific binding of antibodies.
Since the binding of the tyramide label is covalent, tyramides of different dyes can be used in several sequential rounds of TSA staining to detect multiple targets in the same sample.
This tyramide is a conjugate of the water-soluble green fluorescent dye AF 488. AF 488 tyramide is a component of many tyramide signal amplification (TSA) kits. It can be used with any antibody or other molecules (streptavidin, etc.) conjugated to HRP to stain cells and tissues by immunofluorescence methods.

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花青5马来酰亚胺 (Cyanine5 maleimide)
Cyanine5 马来酰亚胺与硫醇反应生成染料标记的缀合物。铜 (II)-TВTA 催化缓冲液,1.5x (Copper(II)-TВTA catalytic buffer, 1.5x)
即用型催化缓冲液含有铜(II) 和 TBTA 配体。适用于核酸和小分子的点击化学修饰。AF 594 tyramide
A tyramide derivative of AF 594, a red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.General properties
Appearance: | orange solid |
Molecular weight: | 856.03 |
Molecular formula: | C41H53N5O11S2 |
Solubility: | soluble in water, DMSO, DMF |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
安全数据表(MSDS): | 下载 |
产品规格 |
Spectral properties
Excitation/absorption maximum, nm: | 495 |
ε, L⋅mol−1⋅cm−1: | 71800 |
Emission maximum, nm: | 519 |
Fluorescence quantum yield: | 0.91 |
CF260: | 0.16 |
CF280: | 0.10 |
