LumiCell Reticulocyte Stain
| 目录号 | 数量 | 价格 | 提前通知时间 | 购买此产品 |
|---|---|---|---|---|
| 17410 | 50 mL | $115 | 现货 |
Reticulocytes are immature red blood cells produced in the bone marrow and released into the peripheral blood, where they mature into erythrocytes. An increase or decrease in reticulocyte count can indicate erythropoiesis activity or failure, especially relative to anemias and bone marrow dysfunction.
In mammals, reticulocytes lack a cell nucleus, like mature erythrocytes, but still contain residual organelles (ribosomes and mitochondria) and residual RNA and DNA, which are absent in mature red blood cells. The nucleic acid dyes, like Thiazole Orange, reveal the reticular (mesh-like) network of ribosomal RNA (rRNA) in reticulocytes, thereby visually differentiating them from mature erythrocytes. Also, the Thiazole Orange stain allows visual differentiating of reticulocyte staging — new cells have more RNA content than mature reticulocytes with low RNA content.
LumiCell Reticulocyte Stain is a ready-to-use solution of Thiazole Orange for determining a count of reticulocytes in human peripheral blood. Thiazole Orange adheres to rRNA and DNA, forming a fluorescent complex with absorption at 509 nm and emission at 532 nm. LumiCell Reticulocyte Stain is suitable both for microscopy and flow cytometry assays.
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sulfo-Cyanine3 amine
Water soluble cyanine dye with bright fluorescence and high extinction coefficient. Contains an amino group for the conjugation with electrophiles and enzymatic transamination labeling.AF 594 tyramide
A tyramide derivative of AF 594, a red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.DAPI, blue fluorescent nucleic acid stain
DAPI (4′,6-diamidino-2-phenylindole) is a blue-emitting fluorescent DNA dye used for chromosome staining and as a nuclear counterstain in fluorescence microscopy and flow cytometry.General properties
| Appearance: | orange solution |
| Molecular weight: | 432.33 |
| Molecular formula: | C19H17IN2S |
| Quality control: | NMR 1H and HPLC-MS (95+%), functional testing |
| Storage conditions: | 12 months after receival at 2-8°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| 安全数据表（MSDS）: | 下载 |
| 产品规格 |
Spectral properties
| Excitation/absorption maximum, nm: | 509 |
| Emission maximum, nm: | 532 |
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